Abstract
A rapid capillary electrophoresis method for the separation of five natural pharmacologically active compounds from extracted Rhodiola, namely salidroside, tyrosol, rhodionin, gallic acid and ethyl gallate has been developed. The separation of five natural pharmacologically active compounds was carried out in a fused-silica capillary with 14 mM boric acid, 30 mM SDS and 2.5% acetonitrile, adjusted to pH 10.7 with NaOH. Applied potential was 21kV. The temperature of the capillary was maintained at 25 °C by the instrument thermostating system, with the correlation coefficients of 0.9805–0.9989 for migration time, and relative standards of < 3.52% for peak areas. The established method is rapid and reproducible for the separation of five natural pharmacologically compounds from extracts of Rhodiola with satisfactory results.
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Acknowledgement.
We would like to thank Ms Yan Cheng and Mr Yongfei Ming for their kindly technical assistance. This work was financed by grants from the “Wester Light” programme of talent cultivation of The Chinese Academy of Sciences (CAS) and Ministry of the Personnel of China and the National Science Foundation under Grant 20075016.
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Suo, Y., Wang, H., Li, Y. et al. Analysis of Five Pharmacologically Active Compounds from Rhodiola for Natural Product Drug Discovery with Capillary Electrophoresis. Chromatographia 60, 589–595 (2004). https://doi.org/10.1365/s10337-004-0415-5
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DOI: https://doi.org/10.1365/s10337-004-0415-5