Abstract
A simple, rapid, and sensitive high-performance liquid chromatographic (HPLC) method coupled with electrospray mass spectrometry (ESI-MS) has been used to determine sanguinarine and chelerythrine in exogenously contaminated honey. Sample extracts were separated on a C8 reversed-phase HPLC column with acetonitrile–acetate buffer (40:60) as mobile phase. After ESI the abundance of protonated molecules was recorded by selected-ion recording (SIR) of m/z 332.5, 348.5, and 356.5 for sanguinarine, chelerythrine, and the internal standard, tetrahydropalmatine, respectively. The internal standard technique was used to construct calibration plots for quantitation of sanguinarine and chelerythrine; the linear ranges were 5.25–1050 and 3.75–750 ng mL−1, respectively, with correlation coefficients of 0.9993 and 0.9989, respectively. The limits of detection for sanguinarine and chelerythrine were 1.60 and 1.11 ng mL−1, respectively.
Similar content being viewed by others
References
Viňas P, Balsalobre N, López-Erroz C, Hernández-Córdoba M (2004) J Chromatogr A 1022:125–129
Martel AC, Zeggane S (2002) J Chromatogr A 954:173–180
Verzegnassi L, Royer D, Mottier P, Stadler RH (2003) Food Addit Contam 20:335–342
Crews C, Startin JR, Clarke PA (1997) Food Addit Contam 14:419–428
Hakim SAE, Mijovie V, Walker J (1961) Nature 189:198–200
Balderstone P, Dyke SF (1977) J Chromatogr 132:359–362
He Y, Du FL, Feng YB, Xie ZM (1999) Nat Prod Res Dev 11:34–37
Bugatti C, Colombo ML, Tome F (1987) J Chromatogr 393:312–316
Han LF, Nowicky W, Guttman V (1991) J Chromatogr 543:123–128
Reinhart P, Harkrader R, Wylie R, Yewey G, Van Horne KC (1991) J Chromatogr 570:425–434
Táborská E, Bochořáková H, Paulová H (1994) Planta Med 380–381
Zeng JG, Zhang S, Hou TZ, Xu X (1999) Chin Trad Med Mater 22:229
An CX, Yang GD, Ye JT, Fu JM (2001) Chin Trad Patent Med 23:824–825
Walterová D, Preininger V, Šimánek V (1984) Planta Med 149–150
Husain S, Narsimha R, Rao RN (1999) J Chromatogr A 863:123–126
Ševčík J, Vičar J, Ulríchová J, Válka I, Lemr K, Šimánek V (2000) J Chromatogr A 866:293–298
Jandera P, Engelhardt H (1980) Chromatographia 13:18–23
Luo XB, Chen B, Yao SZ, Zeng JG (2003) J Chromatogr A 986:73–81
Li W, Gu C, Zhang H, Awang DV, Fitzloff JF, Fong HH, van Breemen RB (2000) Anal Chem 72:5417–5422
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Luo, Xb., Chen, B. & Yao, Sz. High-Performance Liquid Chromatography with Electrospray Mass Spectrometry for Rapid and Sensitive Determination of Sanguinarine and Chelerythrine in Exogenously Contaminated Honey. Chromatographia 60, 347–351 (2004). https://doi.org/10.1365/s10337-004-0391-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-004-0391-9