Abstract
An indirect method is described for determination of phytate in human urine. The method is based on hydrolysis of the phytate and determination of myo-inositol, one of the hydrolysis products. Chromatographic separations were performed on an Aminex HPX-87C column with Milli-Q water as mobile phase; 5 mM ammonium acetate was added post-column. The detector counted positive ions by monitoring m/z=198, which corresponds to the adduct of myo-inositol with the ammonium cation. The relative standard deviations obtained for standards containing 0.5, 1, and 1.5 mg L−1 phytate were 4.1, 3.0, and 2.7% respectively (n=5). The limit of detection was 60 μg L−1. Different urine samples were analyzed both by this method and by an alternative analytical method based on GC–MS. The results from both methods were comparable.
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Perelló, J., Isern, B., Muñoz, J. et al. Determination of Phytate in Urine by High-Performance Liquid Chromatography–Mass Spectrometry. Chromatographia 60, 265–268 (2004). https://doi.org/10.1365/s10337-004-0379-5
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DOI: https://doi.org/10.1365/s10337-004-0379-5