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Determination of Benzimidazole Fungicides by HPLC with Fluorescence Detection After Micellar Extraction

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Abstract

An analytical method was developed to determine the benzimidazole fungicides and their residues (benomyl, carbendazim, thiabendazole and fuberidazole) in real water samples. Analyses were performed by reverse phase (RP) HPLC with direct fluorescence detection with mobile phase methanol:water, 40:60 (v/v) with 0.6% (v/v) ammonia. The extraction of analytes from water samples was performed with the use of micellar systems. Specifically, oligoethylene glycol monoalkyl ether (Genapol X-080) and polyoxyethylene 10 lauryl ether (POLE) were used as extractants. The recoveries of fungicides obtained in spiked water samples ranged from 68% to 94% for Genapol and from 68% to 96% for POLE. The limit of detection (LOD) was lower than 6 μg L−1 for carbendazim, 7 μg L−1 benomyl, 0.15 μg L−1 for thiabendazole and 0.01 μg L−1 for fuberidazole in both surfactants.

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References

  • Garrido Frenich A, Picón Zamora D, Martínez Vidal JL, Martínez Galera M (2003) Anal Chim Acta 477:211–222

    Article  Google Scholar 

  • Rodríguez R, Picó Y, Font G, Mañes J (2001) J Chromatogr A 924:387–396

    PubMed  Google Scholar 

  • Picón Zamora D, Martínez Galera M, Garrido Frenich A, Martínez Vidal JL (2000) Analyst 125:1167–1174

    Article  PubMed  Google Scholar 

  • Fernández Alba AR, Tejedor A, Agüera A, Contreras M, Garrido J (2000) J AOAC Int 83:748–755

    PubMed  Google Scholar 

  • Martínez Galera M, Picón Zamora D, Martínez Vidal JL, Garrido Frenich A, Espinosa Mansilla A, Muñoz de la Peña A, Salinas López F (2003) Talanta 59:1107–1116

    Article  Google Scholar 

  • Bernal JL, del Nozal MJ, Toribio L, Jiménez JJ, Atienza J (1997) J Chromatogr A 787:129–136

    Article  CAS  PubMed  Google Scholar 

  • Tharsis N, Portillo JL, Broto-Puig F, Comellas L (1997) J Chromatogr A 787:95–101

    Article  Google Scholar 

  • Sannino A (1995) Food Chem 52:57–61

    Article  CAS  Google Scholar 

  • Di Muccio A, Girolimetti S, Attard Barbini D, Pelosi P, Generali T, Vergori L, de Merulis G, Leonelli A, Stefanelli P (1999) J Chromatogr A 833:61–65

    Article  PubMed  Google Scholar 

  • Anastassiades M, Schwack W (1998) J Chromatogr A 825:45–54

    Article  CAS  PubMed  Google Scholar 

  • Di Muccio A, Camoni I, Ventriglia M, Attard Barbini D, Mauro M, Pelosi P, Generali T, Ausili A, Girolimetti S (1995) J Chromatogr A 697:145–152

    Article  Google Scholar 

  • Sandahl M, Mathiasson L, Jönsson JA (2000) J Chromatogr A 893:123–131

    Article  CAS  PubMed  Google Scholar 

  • Chiba M, Singh RP (1986) J Agric Food Chem 34:108–112

    CAS  Google Scholar 

  • Padrón Sanz C, Sosa Ferrera Z, Santana Rodríguez JJ (2002) Anal Chim Acta 470:205–214

    Article  Google Scholar 

  • Mahugo Santana C, Sosa Ferrera Z, Santana Rodríguez JJ (2002) Analyst 127:1031–1037

    Article  PubMed  Google Scholar 

  • Halko R, Hutta M (2002) Fresen Environ Bull 11:22–26.

    CAS  Google Scholar 

  • Carabias Martínez R, Rodríguez Gonzalo E, Jiménez MGG, García Pinto C, Pérez Pavón JL, Hernández Mendez J (1996) J Chromatogr A 754:85–96

    Article  Google Scholar 

  • Hinze WL, Pramauro E (1993) CRC Crit Rev Anal Chem 24:133–177

    CAS  Google Scholar 

  • Quina FH, Hinze WL (1999) Ind Eng Chem Res 38:4150–4168

    Article  CAS  Google Scholar 

  • Carabias Martínez R, Rodríguez Gonzalo E, Moreno Cordero B, Pérez Pavón JL, García Pinto C, Fernández Laespada E (2000) J Chromatogr A 902:251–265

    Article  PubMed  Google Scholar 

  • Lindsay S (1992) High Performance Liquid Chromatography, Barnes J (ed) John Wiley and Sons: New York, pp. 71–73

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, Bratislava 842 15, Slovakia

    R. Halko

  2. Department of Chemistry, Faculty of Marine Sciences, University of Las Palmas de G. C., Las Palmas de G.C, 35017, Spain

    C. Padron Sanz, Z. Sosa Ferrera & J. J. Santana Rodríguez

Authors
  1. R. Halko
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  2. C. Padron Sanz
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  3. Z. Sosa Ferrera
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  4. J. J. Santana Rodríguez
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Correspondence to J. J. Santana Rodríguez.

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Halko, R., Sanz, C., Ferrera, Z. et al. Determination of Benzimidazole Fungicides by HPLC with Fluorescence Detection After Micellar Extraction. Chromatographia 60, 151–156 (2004). https://doi.org/10.1365/s10337-004-0364-z

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