Abstract
A simple, rapid and sensitive high-performance liquid chromatographic method with fluorescence detection for the simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline was developed, and successfully applied to the analysis of commercial tetracycline antibiotics. The separation was performed on a reverse-phase C18 column with a gradient elution composed of methanol and sodium acetate buffer (containing disodium ethylenediaminetetraacetate and calcium chloride, pH 8.10) as the mobile phase, and fluorescence detection at 532 nm (excitation at 380 nm). The detection limits for oxytetracycline, doxycycline, tetracycline and chlortetracycline were 0.1, 0.5, 0.3 and 0.4 μg L−1, respectively. Data with respect to precision and accuracy were reported and discussed.
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References
Altmann AE, Beeuwkes H, Brumbacher PJ, Buytendijk HJ, Gijzen AHG, Marsen FPV (1968) Clin Chim Acta 20:185–188
Miller R, Sokoloski TD, Mitscher LA, Bonnaci AC, Hoener BJ (1973) J Pharm Sci 62:1143–1147
Fernandez-Gonzalez R, Garcia-Falcon MS, Simal-Gandara J (2002) Anal Chim Acta 455:143–148. DOI 10.1016/s0003-2670(01)01590-2
Weng ND, Geelen S, Roets E, Hoogmartens J (1990) J Pharm Biomed 8:891–898. DOI 10.1016/0731-7085(90)80138-f
Valcavi U, Brandt A, Corsi GB, Minoja F, Pascucci G (1981) J Antibiot 34:34–39
Chen CL, Gu XL (1995) J AOAC Int 78:1369–1377
Huang TS, Du WX, Marshall MR, Wei CI (1997) J Agr Food Chem 45:2602–2605
Furusawa N (2003) Talanta 59:155–159. DOI 10.1016/s0039-9140(02)00472-1
Ding XJ, Mou SF (2000) J Chromatogr A 897:205–214. DOI 10.1016/s0021-9673(00) 00779-2
Cinquina AL, Longo F, Anastasi G, Giannetti L, Cozzani R (2003) J Chromatogr A 987:227–233. DOI 10.1016/s0021-9673(02)01446-2
Furusawa N (2001) Chromatographia 53: 47–50
Zurhelle G, Muller-Seitz E, Petz M (2000) J Chromatogr B 739:191–203. DOI 10.1016/s0378-4347(99)00477-6
Lindsey ME, Meyer M, Thurman EM (2001) Anal Chem 73:4640–4646.
Kazemifard AG, Moore DE (1997) J Pharm Biomed 16:689–696. DOI 10.1016/s0731-7085(97)00089-7
Vienneau DS, Kindberg CG (1997) J Pharm Biomed 16:111–117. DOI 10.1016/s0731-7085(97)00009-5
McCracken RJ, John W, Blanchflower WJ, Haggan SA, Kennedy DG (1995) Analyst 120:1763–1766.
Iwaki K, Okumura N, Yamazaki M (1992) J Chromatogr 623:153–158. DOI 10.1016/0021-9673(92)85310-p
Croubels S, Baeyens W, Vanpeteghem C (1995) Anal Chim Acta 303:11–16. DOI 10.1016/0003-2670(94)00461-t
Yoneda Y, Furushiro N, Andoh R (1989) J Food Hyg Soc Jpn 30:42
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Lu, HT., Jiang, Y., Li, HB. et al. Simultaneous Determination of Oxytetra- cycline, Doxycycline, Tetracycline and Chlortetracycline in Tetracycline Antibiotics by High-Performance Liquid Chromatog- raphy with Fluorescence Detection. Chromatographia 60, 259–264 (2004). https://doi.org/10.1365/s10337-004-0356-z
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DOI: https://doi.org/10.1365/s10337-004-0356-z