Abstract
A sensitive and selective method for quantitation of glimepiride in human plasma was established using liquid chromatography-electrospray ionization tandem mass spectrometry. Three different methods for the sample preparation of glimepiride and an internal standard were investigated (liquid-liquid extraction, solid-phase extraction and protein precipitation). Glipizide was used as an internal standard. Compounds were separated on a C18 column with 80% acetonitrile and 20% deionized water (adjusted to pH 3.5 with acetic acid), as mobile phase at a flow rate of 200 μL min−1. By use of multiple reaction monitoring mode in MS-MS with liquid-liquid extraction and solid-phase extraction, glimepiride and glipizide were detected without severe interference from the human plasma matrix. Glimepiride produced a protonated precursor ion ([M+H]+) at m/z 491 and a corresponding product ion at m/z 352, and the internal standard produced a protonated precursor ion ([M+H]+) at m/z 446 and a corresponding product ion at m/z 321. The limit of quantitation was 0.1 ng mL−1, 0.5 ng mL−1 and 1.0 ng mL−1 when using liquid-liquid extraction, solid-phase extraction and protein precipitation, respectively. The validation, reproducibility, stability, and recovery of the different sample preparation methods were comparable and all the methods gave reliable results. The method has been successfully applied to pharmacokinetic study of glimepiride in human plasma.
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References
Draeger E (1995) Diabet Res Clin Prac 28:139–146
Müller G, Geisen K (1996) Horm Metab Res 28:469–487
Eckert H, Keliner D, Gantz H (1993) Clin Rep 27:1493–1524
Kolterman Q, Gray RS (1984) Diabets 32:346–354
Drager KE (1996) Horm Metab Res 28:419–425
Sonenberg GE (1997) Am Pharmacother 37:671–676
Nunez M, Ferguson JE, Machacek D, Jacob G, Oda RP, Lawson GM, Landers JP (1995) Anal Chem 67:3668–3675
Roche ME, Oda RP, Lawson GM, Landers JP (1997) Electrophoresis 18:1865–1874
Drummer OH, Kotsos A, McIntyre IM (1993) J Anal Toxicol 17:225–229
Lo DS, Chao TC, Ng Ong SE, Yao YJ, Koh TH (1997) Forensic Sci Int 90:205–214
Lehr K, Damm P (1990) J Chromatogr 526:497–505
Altinöz S, Tekeli D (2001) J Pharm Biomed Anal 24:507–515
Maurer HH, Kratzsch C, Kraemer T, Peters FT, Weber AA (2002) J Chromatogr B 773:63–73
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Kim, H., Chang, K., Park, C. et al. Determination of Glimepiride in Human Plasma by LC-MS-MS and Comparison of Sample Preparation Methods for Glimepiride. Chromatographia 60, 93–98 (2004). https://doi.org/10.1365/s10337-004-0351-4
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DOI: https://doi.org/10.1365/s10337-004-0351-4