Skip to main content
SpringerLink
Log in

HPLC Separation of O-Acylated Flavonoids and Biflavones from Some Species of Gymnospermae

  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Flavonoids present in the extracts from leaves of Pseudotsuga menziesii (Pinaceae), Ginkgo biloba (Ginkgoaceae) and Podocarpus dacrydioides (Podocarpaceae) were separated by use of the reversed phase HPLC method. The analysed compounds belong to different groups of flavonoids – biflavones (amentoflavone, bilobetin, 5’−methoxybilobetin, podocarpusflavone A, sequoiaflavone, podocarpusflavone B, ginkgetin, isoginkgetin, sciadopitysin, kayaflavone, hinokiflavone, 2,3−dihydrosciadopitysin, 2,3−dihydroisoginkgetin), O−acylated flavonol glycosides (daglesiosides I, II, III, IV, trans−tiliroside, trans−ditiliroside), flavonol O−glycosides (astragalin, isoquercetin) and flavonol aglycones (kaempferol, quercetin, isorhamnetin). The conditions for flavonoid separation were optimized using various RP−18 columns. The chromatographic resolution was performed with isocratic or gradient elution – optimized by Drylab program or by traditional “trial-and-error” method, depending on the composition of flavonoid complex.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Geiger H (1993) In: Harborne JB (ed) The flavonoids: Advances in research since 1986. Chapman and Hall, London

  • Krauze-Baranowska M, Sowiński P (1999) Planta Med 65:482−484

    CAS  Google Scholar 

  • Krauze-Baranowska M, Bączek T, Kaliszan R (2001) In: Lead compounds from higher plants. International Symposium of the Phytochemical Society of Europe, Switzerland, Lausanne

  • Roy SK, Quasim MA, Kamil M, Ilyas M (1987) Phytochemistry 26:1985–1987

    Article  CAS  Google Scholar 

  • Krauze-Baranowska M, Sowiński P (2003) J Nat Prod submitted

  • Niemann GJ (1988) In: Harborne JB (ed) The flavonoids. Chapman and Hall, London

  • Tang Y, Lou F, Wang J, Li Y, Zhuang S (2001) Phytochemistry 58:1251–1256

    Article  CAS  PubMed  Google Scholar 

  • Dellus V, Milla IN, Scalbert A, Menard C, Michon V, Herve du Penhoat CLM (1997) Phytochemistry 45:1573–1578

    Article  CAS  Google Scholar 

  • Foo LY, Helm R, Karchesy J (1992) Phytochemistry 31:1444–1445

    Article  CAS  Google Scholar 

  • Johnson T (1999) Ethnobotany desk reference. CRC Press, New York

  • Lewis NG, Davin LB (1999) In: Sankawa U (ed) Comprehensive natural products chemistry. Elsevier, Amsterdam

  • Giese LA (2001) Gastroent Nursing 24:95–97

    Article  CAS  Google Scholar 

  • Sharma G, Singh RP, Agarwal R (2003) Anticancer Res 23:2649–2655

    CAS  PubMed  Google Scholar 

  • Krauze-Baranowska M (2002) Acta Pol. Pharm 59:403–410

    CAS  Google Scholar 

  • van Beek TA, Bombardelli E, Morazzoni P, Peterlongo F (1998) Fitoterapia 69:195–224

    Google Scholar 

  • Kanowski S (1997) Münch Med Wochenschr 139:47–50

    Google Scholar 

  • Dongen N, Rossum E, Kessels A, Sielhorst H, Knipschild P (2003) J Clin Pharmacol 56:367–76

    Article  Google Scholar 

  • Sticher O (1992) Planta Med 59:2–11

    Google Scholar 

  • Hasler A, Sticher O, Meier B (1992) J Chromatogr 605:41–48

    Article  CAS  Google Scholar 

  • van Beek TA (2002) J Chromatogr A 967:1–55

    PubMed  Google Scholar 

  • Gobato S, Griffini A, Lolla E, Peterlongo F (1996) Fitoterapia 67:152–158

    Google Scholar 

  • Krauze-Baranowska M, Cisowski W, Wiwart M, Madziar B (1999) Planta Med 65:572–573

    CAS  PubMed  Google Scholar 

  • Krauze-Baranowska M (2003) Z Naturforsch c, submitted

  • Krauze-Baranowska M (2004) Z Naturforsch 59c:43–47

    Google Scholar 

  • Bączek T, Lewandowska G, Kaliszan R, Krauze-Baranowska M, Cisowski W (2001) LC-GC Europe 2:25–33. http://lcgeurope.advpe.adv100.com/lcgceurope/data/articlestandard/lcgeurope/512001/5427/article.pdf

    Google Scholar 

  • Bucar F, Jackak SM, Noreen Y, Kartnig T, Perera P, Bohlin L, Schubert-Zsilavecz M (1997) Planta Med 64:373–374

    Google Scholar 

  • Ma SC, But PPH, Ooi VVC, He YH, Lee SHS, Lee SF, Lin RC (2001) Biol Pharm Bull 24:311–312

    Article  CAS  PubMed  Google Scholar 

  • Neue UD (1997) HPLC columns theory, technology and practice. Wiley Chichester

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacognosy, Medical University of Gdańsk, Gen. J. Hallera 107 str, 80-416, Gdańsk, Poland

    M. Krauze-Baranowska & D. Głód

  2. Department of Pharmacology and Biopharmacy, Medical University of Gdańsk, Gen. J. Hallera 107 str, 80-416, Gdańsk, Poland

    T. Baczek & R. Kaliszan

  3. Institut für Botanik der TU Darmstadt, Schnittspahnstr. 3, 64287, Darmstadt, Germany

    E. Wollenweber

Authors
  1. M. Krauze-Baranowska
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Baczek
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Głód
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Kaliszan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. Wollenweber
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Krauze-Baranowska.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Krauze-Baranowska, M., Baczek, T., Głód, D. et al. HPLC Separation of O-Acylated Flavonoids and Biflavones from Some Species of Gymnospermae. Chromatographia 60, 9–15 (2004). https://doi.org/10.1365/s10337-004-0349-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-004-0349-y

Keywords

Search

Navigation