Abstract
An alternative and practical method is described for simultaneous detection and quantification of the potent hallucinogen lysergic acid diethylamide (LSD) and related compounds in urine and serum samples. The procedure is based on liquid–liquid re-extraction with ethyl acetate and reversed-phase liquid chromatography coupled with fluorescence detection (HPLC–FLD). With detection limits in urine and serum samples of ca 0.07 ng mL−1 for LSD, nor-LSD, and iso-LSD, respectively, the method is well suited to forensic investigations. Application of the method to clinical samples and autopsy material enable selective identification and accurate quantification of LSD and related compounds. Comparison of results with those obtained from an LSD immunoassay (EMIT II) emphasize the need for chromatographic confirmation.
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References
Foye WH, Lemke TL, Williams DA (1995) Principles of medicinal chemistry, 4th edn. William and Wilkins, Baltimore
Baselt RC (2000) Disposition of toxic drugs and chemicals in man, 5th edn. Chemical Toxicology Institute, Foster City
Geschwinde T (1998) Rauschdrogen— Marktformen und Wirkungsweisen, 4th edn. Springer, Berlin
Novak SJ (1998) Endeavour 21(1):21–23
Nelson CC, Foltz RL (1992) Anal Chem 64:1578–1584
Poch GK, Klette KL, Anderson C (2000) J Anal Toxicol 24:170–179
Gesetz über den Verkehr mit Betäubungsmitteln (Betäubungsmittelgesetz, BtMG), 01031994 (BGBl I, 358), Germany
Upshall DG, Wailling DG (1972) Clin Chim Acta 36:67–73
Altunkaya D, Smith RN (1990) Forensic Sci Int 47:113–121
Cody JT, Valtier S (1997) J Anal Toxicol 21:459–464
Wu AHB, Feng XY, Pajor A, Gornet GT, Wong SS, Forte E, Brown J (1997) J Anal Toxicol 21:181–184
Wiegand RF, Klette KL, Stout PR, Gehlhausen JM (2002) J Anal Toxicol 26:519–523
Maurer HH (1992) J Chromatogr 580:3–41
Sklerov JH, Kalasinsky KS, Ehorn CA (1999) J Anal Toxicol 23:474–478
Musshoff F, Daldrup T (1997) Forensic Sci Int 88:133–140
Libong D, Bouchonnet S, Ricordel I (2003) J Anal Toxicol 27:24–29
Burnley BT, George S (2003) J Anal Toxicol 27:249–252
McCarron MM, Walberg CT, Baselt RC (1990) J Anal Toxicol 14(3):165–167
Bergemann D, Geier A, von Meyer L (1999) J Forensic Sci 44(2):372–374
Röhrich J, Zörntlein S, Becker J (2000) Forensic Sci Int 107:181–190
Grobosch T, Lemm-Ahlers U (2002) J Anal Toxicol 26:181–186
Frost M, Kohler H, Blaschke G (1997) J Chromatogr B 693(2):313–319
Hoja H, Marquet P, Verneuil P, Lotfi H, Dupuy JL, Lachatre G (1997) J Chromatogr B 692(2):329–335
Cougnard T, Charlier C, Plomteux G (1999) Toxicorama 11(2):99–102
White SA, Kidd AS, Webb KS (1999) J Forensic Sci 44(2):375–379
Bodin K, Svensson JO (2001) Ther Drug Monit 23(4):389–393
Canezin J, Cailleux A, Turcant A, Le Bouil A, Harry P, Allain P (2001) J Chromatogr B 765(1):15–27
Klette KL, Horn CK, Stout PR, Anderson CJ (2002) J Anal Toxicol 26:193–200
Skopp G, Pötsch L, Mattern R, Aderjan R (2002) Clin Chem 48(9):1615–1618
Horn CK, Klette KL, Stout PR (2003) J Anal Toxicol 27:459–463
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Revised: 1 December 2003 and 9 February 2004
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Pietsch, J., Schulz, K., Körner, B. et al. Alternative Method for Forensic Determination of Lysergic Acid Diethylamide and Related Compounds in Body Fluids by Liquid–Liquid Extraction and HPLC with Fluorescence Detection. Chromatographia 60, 89–92 (2004). https://doi.org/10.1365/s10337-004-0344-3
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DOI: https://doi.org/10.1365/s10337-004-0344-3