Abstract
A comparative study of the retention behaviour of disubstituted benzene derivatives on (2-hydroxy-3-methacryloyloxypropyl β-cyclodextrin-co-N-vinylpyrrolidone) copolymers physically adsorbed onto porous silca gel was achieved. Under reversed-phase mode, the separation process is based on an inclusion complex formation, and the inclusion selectivity is strongly influenced by hydrogen bonds and/or steric effects. The influence of cyclodextrin derivatives used as mobile phase additives for the conventional reversed-phase high-performance liquid chromatography was also examined with the aim to confirm the formation of inclusion complexes between CD derivatives and test molecules. Under normal-phase mode, it was found that the retention and separation processes are mainly controlled by hydrophilic interactions between the polar substituents of the solute and the hydroxyl groups of the cyclodextrin units. Finally, the enantiomer separation ability was briefly demonstrated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kawaguchi Y, Tanaka M, Nakae M, Funaso K, Sheno T (1983) Anal Chem 55 (1):1852–1857
Hefnawy M, Stewart J (1998) Anal Lett 31 (4):659–667
Pawlowska M, Chen S, Armstrong DW (1993) J. Chromatogr 641:257–265
Sébille B, Guillaume M, Vidal-Majar C, Thuaud N (1997) Chromatographia 45:383–389
Tanaka M, Shono T, Zhu D-Q, Kawaguchi Y (1989) J. Chromatogr 469:429–433
Tanaka M, Kawaguchi Y, Shono T (1983) J Chromatogr 267:285–292
Tanaka M, Kawaguchi Y, Niinae T, Shono T (1984) J Chromatogr 314:193–200
Armstrong DW, Chang LW, Wang X, Ibrahim H, Reit III GR, Beesley TE (1997) J Liq Chromatogr Rel Techno 20:3279–3295
Stalcup A, Chang S, Armstrong DW, Pitha J (1990) J Chromatogr 513:181–194
Janus L, Carbonnier B, Morcellet M, Ricart G, Crini G, Deratani A (2003) Macromol Biosci 3:198-209
Janus L, Carbonnier B, Deratani A, Crini G, Laureyns J, Morcellet M (2003) New J Chem 27:307–312
Carbonnier B, Janus L, Lekchiri Y, Morcellet M (2004) J Chrom Sci 42:37–42.
Tang J, Cline Love L (1997) Anal Chim Acta 344:37–143
Armstrong DW, DeMond W, Alak A, Hinze WL, Riehl TE, Bui KH (1985) Anal Chem 57-234–237
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Carbonnier, B., Janus, L. & Morcellet, M. Evaluation of Inclusion Selectivity of (2-hydroxy-3-methacryloyloxypropyl β-cyclodextrin-co-N-vinylpyrrolidone) Copolymers Coated Silica Stationary Phase by High Performance Liquid Chromatography. Chromatographia 60, 109–112 (2004). https://doi.org/10.1365/s10337-004-0334-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-004-0334-5