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Catalytic and DNA-Hydrolyzing Activities of Purified Immunoglobulins IgG from Patients Sera with Autoimmune Disease by Pseudobiospecific Chromatography Using Histidyl-Aminohexyl-Sepharose

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Abstract

Catalytic autoimmune antibodies from patients with antiphospholipid (aPL) antibodies were purified using histidyl-aminohexyl-sepharose gel. The sera were loaded on the columns equilibrated with 25 mM MOPS buffer pH 7.4 and the absorbed proteins were eluted by adding 0.2 M NaCl in the equilibrating buffer. Antibodies purity was evaluated by SDS-PAGE. The purified immunoglobulins G from patients with (aPL) sera by histidyl-aminohexyl-sepharose show DNA-degrading activity of the plasmid pUC19 DNA and catalytic activity in hydrolyzing the peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin.

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Authors and Affiliations

  1. Department of Medical Biology, Faculty of Pharmacy, Lebanese University, P.O.Box. 14/6573, Beirut, Lebanon

    A. Elkak

  2. The National Council for Scientific Research, P.O.Box. 11/8281, Beirut, Lebanon

    A. Elkak

  3. Laboratoire d’Interactions Moléculaires et de Technologie des séparations, Université de Technologie de Compiègne, B.P. 20569, 60205, Compiègne, France

    M. Bourhim, Y. Coffinier & M. A. Vijayalakshmi

Authors
  1. A. Elkak
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  2. M. Bourhim
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  3. Y. Coffinier
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  4. M. A. Vijayalakshmi
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Correspondence to A. Elkak.

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Elkak, A., Bourhim, M., Coffinier, Y. et al. Catalytic and DNA-Hydrolyzing Activities of Purified Immunoglobulins IgG from Patients Sera with Autoimmune Disease by Pseudobiospecific Chromatography Using Histidyl-Aminohexyl-Sepharose. Chromatographia 60, 165–169 (2004). https://doi.org/10.1365/s10337-004-0333-6

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  • DOI: https://doi.org/10.1365/s10337-004-0333-6

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