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A New Bioautographic Screening Method for the Detection of Estrogenic Compounds

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Abstract

The yeast estrogen screen was introduced as biological detection method for high performance thin layer chromatography. Yeast cells were grown directly on HPTLC plates, where in the presence of estrogenic substances the production of the enzyme β-galactosidase was induced. For the natural ligand 17 β-estradiol, sensitivity could be improved by a factor of 20 using the fluorogenic substrate 4-methylumbelliferyl β-D-galactopyranoside instead of the chromogenic substrate chlorophenol red-β-D-galactopyranoside. The fluorescence intensity of “estrogenic” zones was measured using a commercial TLC-Scanner. A nearly full dose-response curve was obtained for 17 β-estradiol masses between 2.75 and 550 pg, or concentrations of 2.75 to 550 μg L−1.

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Authors and Affiliations

  1. Engler-Bunte-Institut, Bereich Wasserchemie, Universität Karlsruhe (TH), Engler-Bunte-Ring 1, 76131, Karlsruhe, Germany

    M. B. Müller, C. Dausend & F. H. Frimmel

  2. Landesanstalt für Umweltschutz des Saarlandes (LfU), Malstatter Str. 17, 66117, Saarbrücken, Germany

    C. Weins

Authors
  1. M. B. Müller
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  2. C. Dausend
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  3. C. Weins
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  4. F. H. Frimmel
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Correspondence to F. H. Frimmel.

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Müller, M., Dausend, C., Weins, C. et al. A New Bioautographic Screening Method for the Detection of Estrogenic Compounds. Chromatographia 60, 207–211 (2004). https://doi.org/10.1365/s10337-004-0315-8

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  • DOI: https://doi.org/10.1365/s10337-004-0315-8

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