Abstract
A capillary zone electrophoresis method modified by β-CD was, for the first time, developed for the simultaneous determination of five anthraquinones (physcion, chrysophanol, aloe-emodin, emodin and rhein) in rheum and rheum-containing preparations. The factors relevant to the run buffer media and their effects on the separation were studied. A buffer solution composed of 15 mM sodium borate, 30 mM β-CD, 20% acetonitrile and 1.0% ethanediol was found to be the most suitable electrolyte for separation, whereby the contents of the five anthraquinones in rheum and rheum-containing preparations could be easily determined in 20 min at 20 kV. The relative standard deviation for the determination of the five constituents in the samples varied form 0.16–5.47%, and the recovery ranged between 92.0 and 107.3%. Moreover the inclusion constants of the target compounds, except for rhein with β-CD, were also determined.
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Li, F., Cao, QE. & Ding, Z. Separation and Determination of Five Anthraquinones in Rheum and Rheum-Containing Preparations by Capillary Zone Electrophoresis. Chromatographia 59, 753–757 (2004). https://doi.org/10.1365/s10337-004-0314-9
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DOI: https://doi.org/10.1365/s10337-004-0314-9