Abstract
High-performance liquid chromatographic methods have been developed for stereoselective separations of terfenadine and its active metabolite fexofenadine. Satisfactory enantioseparations of racemic terfenadine were achieved on a Chiralcel column by normal phase elution. Analysis of racemic fexofenadine, as such, took a very long time and achieved poor enantioselectivity on this column; nevertheless, the analyte when derivatised with diazomethane was well resolved. Racemic fexofenadine was also derivatised using a chiral agent: R-(+)-1-phenylethylisocyanate and subjected to achiral LC on a reversed-phase analytical column. Complete enantioseparations were achieved in short analysis times; excess reagent eluted before the diastereoisomeric pair and did not interfere.
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Part of this work was presented at the 10th International Meeting on Recent Developments in Pharmaceutical Analysis RDPA’03, Cogne, Valle d’ Aosta, Italy. June 28–July 1, 2003.
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Rustichelli, C., Gamberini, M., Ferioli, V. et al. Enantioselective Analyses of Antihistaminic Drugs by High-Performance Liquid Chromatography. Chromatographia 60, 99–103 (2004). https://doi.org/10.1365/s10337-004-0310-0
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DOI: https://doi.org/10.1365/s10337-004-0310-0