Abstract
The HPLC separation of enantiomers of underivatized non-protein amino acids was investigated by using a column packed with octadecylsilanized silica coated with N,S-dioctyl-D-penicillamine as a chiral ligand-exchange phase (Sumichiral OA-5000). Good enantiomeric separations were achieved with a variety of α-amino acids carrying aliphatic or aromatic side chains, cyclic imino acids, and β-amino acids, together with α-methyl-α-amino acids, by optimizing the amount (0–20%, v/v) of 2-propanol as the organic component and the concentration (1–5 mM) of Cu2+ as the complexing metal ion in the aqueous-organic eluent.
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Miyazawa, T., Minowa, H., Imagawa, K. et al. Separation of Enantiomers of Non-Protein Amino Acids by High-Performance Liquid Chromatography on a Chiral Ligand-Exchange Column. Chromatographia 60, 45–50 (2004). https://doi.org/10.1365/s10337-004-0298-5
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DOI: https://doi.org/10.1365/s10337-004-0298-5