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Characterization and Diversity of Cyano- bacterial Hepatotoxins (Microcystins) in Blooms from Polish Freshwaters Identified by Liquid Chromatography-Electrospray Ionisation Mass Spectrometry

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Abstract

Microcystins were determined in 36 cyanobacterial (blue-green algal) bloom samples dominated by Microcystis aeruginosa, Aphanizomenon flos-aquae and Nostoc spp. The analysed samples were collected from 8 water bodies in Poland during summers of 1996–2001. Ten microcystins (MC), three major and seven minor variants, were detected in natural cyanobacterial samples by high-performance liquid chromatography (HPLC). The structures of the microcystins were assigned on the basis of liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS). The main microcystins detected in the blooms from Polish freshwaters were MC-LR, MC-RR and MC-YR. Microcystin content of the blooms varied from a few μg g−1 to 1687 μg g−1 freeze-dried material. Also some monodesmethyl (dm) and didesmethyl (dmdm) variants of MC-LR, MC-RR, MC-YR were found in a few analysed samples. Furthermore, two compounds were tentatively characterised as MC-(H4)YR and dm-MC-FR.

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Authors and Affiliations

  1. Department of Applied Ecology, University of Lodz, Banacha 12/16, 90-237, Lodz, Poland

    T. Jurczak & M. Tarczyñska

  2. International Centre for Ecology, Polish Academy of Sciences, Konopnickiej 1, Lomianki, Poland

    T. Jurczak

  3. Department of Biochemistry and Pharmacy, Åbo Akademi University, P.O. Box 66, 20521, Turku, Finland

    K. Karlsson & J. Meriluoto

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  1. T. Jurczak
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  2. M. Tarczyñska
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  3. K. Karlsson
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  4. J. Meriluoto
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Correspondence to T. Jurczak.

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Jurczak, T., Tarczyñska, M., Karlsson, K. et al. Characterization and Diversity of Cyano- bacterial Hepatotoxins (Microcystins) in Blooms from Polish Freshwaters Identified by Liquid Chromatography-Electrospray Ionisation Mass Spectrometry. Chromatographia 59, 571–578 (2004). https://doi.org/10.1365/s10337-004-0279-8

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  • DOI: https://doi.org/10.1365/s10337-004-0279-8

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