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Application of Iodine-Azide Reaction as Postcolumn Reaction in HPLC for Determination of 2-Thiobarbituric Acid

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Abstract

The reaction between iodine and azide ion induced by 2-thiobarbituric acid (TBA) has been utilized as a postcolumn reaction for chromatographic determination of this sulphur compound. The method is based on the separation of thiobarbituric acid on an Nova-Pak® CN HP column with an acetonitrile–aqueous solution of sodium azide mobile phase. The separation stage is followed by spectrophotometric measurement of the residual iodine (λ=350 nm) from the postcolumn iodine-azide reaction induced by thiobarbituric acid after mixing iodine solution containing iodide with the column effluent containing azide ions and the inductor. Chromatograms obtained for thiobarbituric acid showed negative peaks as a result of the decrease in absorbance of background. The detection limit (defined as S/N=3) was 0.16 pmol (22.9 pg) for thiobarbituric acid. Calibration graphs, plotted as peak heights or peak area vs. concentrations, were linear up to 1 nM.

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References

  • Suttnar J, Masova L, Dyr JE (2001) J Chromatogr B, 751:193

  • Fenaille F, Mottier P, Turesky RJ, Ali S, Guy PA (2001) Chromatogr A, 921:237

  • Kurzawa Z, Dobrzańska-Jaszczyk A (1974) Chem. Anal. (Warsaw), 19:1071

  • Kurzawa J (1985) Anal Chim Acta, 173:343

  • Kurzawa J, Kurzawa Z (1986) Chem. Anal. (Warsaw) 31:45

  • Miura Y, Fukasawa K, Koh TJ (1998) J Chromatogr A 804:143

    Article  CAS  Google Scholar 

  • Miura Y, Watanabe MJ (2001) J. Chromatogr. A, 920:163

  • Kurzawa J (1987) Chem Anal (Warsaw), 32:875

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Authors and Affiliations

  1. Department of Instrumental Analysis, University of Łódź, Pomorska, 163, 90-236 Łódź, Poland

    R. Zakrzewski & W. Ciesielski

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  1. R. Zakrzewski
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  2. W. Ciesielski
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Correspondence to R. Zakrzewski.

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Zakrzewski, R., Ciesielski, W. Application of Iodine-Azide Reaction as Postcolumn Reaction in HPLC for Determination of 2-Thiobarbituric Acid. Chromatographia 59, 683–689 (2004). https://doi.org/10.1365/s10337-004-0274-0

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  • DOI: https://doi.org/10.1365/s10337-004-0274-0

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