Abstract
Salmeterol is a β2-adrenergic agonist and an Association of Racing Commissioners International (ARCI) class 3 drug. Trade names of its xinafoate salt are Arial (Dompé), Salmetedur (Menarini), and Serevent (Glaxo). Salmeterol is routinely used to increase ease of breathing in race horses during their training. Due to its bronchodilating and central nervous system stimulant properties, its administration to a horse just prior to race time has the potential to affect the horse’s performance, therefore a reliable method of analysis for this compound is necessary. This paper describes a method for the identification and quantitation of salmeterol in equine urine using liquid-liquid extraction followed by liquid chromatography and tandem mass spectrometry (LC-MS-MS). Urine salmeterol concentrations peaked at about 2 h post-dose following administration of 500 ug both intravenously and intratracheally at concentrations of 14 ng mL−1 and 4 ng mL−1, respectively. Serum concentrations at 30 min were below the minimum level of quantitation.
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Calverley P, Pauwels R, Vestbo J, Jones P, Pride N, Gulsvik A, Anderson J, Maden C (2003) Lancet 361:449–456
Dal Negro RW, Pomari C, Tognella S, Micheletto C (2003) Pulm Pharmacol Ther 16:241–246
Zimmermann T, Gulyas A, Bauer CP, Steinkamp G, Trautmann M (2003) Eur J Med Res 8:428–434
Coraux C, Kileztky C, Polette M, Hinnrasky J, Zahm JM, Devillier P, De Bentmann S, Puchelle E (2003) Oct 3 [Epub ahead of print]
Lindqvist A, Karjalainen EM, Laitinen LA, Kava T, Altraja A, Pulkkinen M, Halme M, Laitinen A (2003) J Allergy Clin Immunol 112:23–28
Lyseng-Williamson KA, Plosker GL (2003) Pharmacoeconomics 21:951–989
Reid DW, Ward C, Wang N, Zheng L, Bish R, Orsida B, Walters EH (2003) Eur Respir J 21:994–999
Henrikson SL, Rush BR (2001) J Am Vet Med Assoc 218:1961–1965
Tong HH, Shekunov BY, York P, Chow, AH (2003) Pharm Res 20:1423–14299
Merck Index (1999) Merck and Company, Inc., Whitehouse Station, NJ
Karpiesiuk W, Lehner A, Tobin T (2003) (submitted for publication 15th International Conference of Racing Analysts and Veterinarians)
Combie J, Blake JW, Nugent TE, Tobin T (1982) Clin Chem 28:83–86
Lehner AF, Hughes CG, Harkins JD, Nickerson C, Mollett B, Dirikolu L, Bosken JM, Camargo F, Boyles J, Troppmann A, Karpiesiuk W, Woods WE, Tobin T (2004) J Anal Tox, in press
Lehner AF, Harkins JD, Karpiesiuk W, Woods WE, Robinson NE, Dirikolu L, Fisher M, Tobin T (2001) J Anal Toxicol 25:280–287
Van Eenoo P, Deventer K, Delbeke FT (2002) Rapid Commun Mass Spec 16:1755–1759
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Publication # 347 from the Equine Pharmacology, Experimental Therapeutics and Toxicology Program at the Maxwell H. Gluck Equine Research Center and the Department of Veterinary Science, University of Kentucky.
Published as Kentucky Agricultural Experiment Station Article 04-14-011 with approval of the Dean and Director, College of Agriculture and Kentucky Agricultural Experiment Station.
The data presented in this paper, in part, was presented at the 15th International Conference of Racing Analysts and Veterinarians, Dubai, UAE, March 2004
In Honour of Professor Edward Houghton: A Celebration of 30 Years in Racing Chemisty
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Lehner, A., Hughes, C., Karpiesiuk, W. et al. Determination of Salmeterol in Equine Urine and Serum. Chromatographia 59 (Suppl 1), S79–S84 (2004). https://doi.org/10.1365/s10337-004-0249-1
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DOI: https://doi.org/10.1365/s10337-004-0249-1