Abstract
A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric method (LC-MS-MS) for the determination of eupatilin in human plasma has been developed. Eupatilin and an internal standard; (S)-N-(3-{3-fluoro-4-[6-(1-methyl-1H-tetrazol-5-yl)-pyridine-3-yl]-phenyl}-2-oxo-oxazolidin-5-ylmethyl)-acetamide (DA-7867) were extracted from human plasma by liquid-liquid extractionand analyzed on a phenyl-hexyl column using the mobile phase: acetonitrile-ammonium formate (10 mM, pH 3.0) (60:40, ν/ν). Analytes were detected using electrospray ionization-tandem mass spectrometry in multiple-reaction monitoring mode. The calibration curve was linear (r = 0.999) over the concentration range: 1.00–500 ng mL−1 with a lower limit of quantification of 1.0 ng mL−1 using a 100 μL plasma sample. The precision (CV%) of this assay ranged: 2.4–7.0%, relative error: −7.0 to +2.0%. Recoveries of eupatilin ranged: 64.3–65.0%, with that of DA-7867 (internal standard) being 87.0 ± 5.3%.
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Koshihara Y, Neichi T, Murota S, Lao A, Fujimoto Y, Tatsuno T (1983) FEBS Lett 1158:41–44
Ahn BO, Ryu BK, Ko JI, Oh TY, Kim SH, Kim WB, Yang J, Lee EB, Hahm KB (1997) J Appl Pharmacol 5:165–173
Oh TY, Ryu BK, Ko JI, Ahn BO, Kim SH, Kim WB, Lee EB, Jin JH, Hahm KB (1997) Arch Pharm Res 20:414–419
Oh TY, Ahn BO, Ko JI, Ryu BK, Son MW, Kim SH, Kim WB, Lee EB (1997) J Appl Pharmacol 5:202–210
Ahn BO, Oh TY, Ryu BK, Kim SH, Kim WB, Kang SH, Chun MS, Yoon JH (1998) J Appl Pharmacol 6:37–44
Hahm KB, Kim JH, You BM, Kim YS, Cho SW, Yim H, Ahn BO, Kim WB (1998) Pancreas 17:153–157
Ahn BO, Ko KH, Oh TY, Cho H, Kim WB, Lee KJ, Cho SW, Hahm KB (2001) Int J Colorectal Dis 16:174–181
Lee JS, Oh TY, Ahn BO, Cho H, Kim WB, Kim YB, Surh YJ, Kim HJ, Hahm KB (2001) Mutat Res 480–481:189–200
Oh TY, Lee JS, Ahn BO, Cho H, Kim WB, Kim YB, Surh YJ, Cho SW, Lee KM, Hahm KB (2001) Gut 49:364–371
Seo HJ, Surh YJ (2001) Mutat Res 496:191–198
Hahm KB, Lee KM, Kim YB, Hong WS, Lee WH, Han SU, Kim MW, Ahn BO, Oh TY, Lee MW, Green J, Kim SJ (2002) Alimen Pharmacol Ther 16 (Suppl. 2):115–127
Ji HY, Lee HW, Shim HJ, Kim SH, Kim WB, Lee HS, Biomed Chromatogr in press.
Kim SH, Kim SH, Lee SD, Kim WB, Lee MG, Kim ND (1997) Res Commun Mol Pathol Pharmacol 97:167–172
Guidance for Industry-Bioanalytical Method Validation, US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, Center for Veterinary Medicine, May 2001, Http://www.fda.gov/cder/guidance/index.htm
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Won Lee, H., Young Ji, H., Hyun Kim, H. et al. Determination of Eupatilin in Human Plasma by High-Performance Liquid Chromatography–Tandem Mass Spectrometry. Chromatographia 59, 425–428 (2004). https://doi.org/10.1365/s10337-004-0221-0
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DOI: https://doi.org/10.1365/s10337-004-0221-0