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Gas Chromatographic – Mass Spectrometric Cardiotonic Glycosides Detection in Equine Urine Doping Analysis

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Abstract

A screening method for the detection of cardiotonic glycosides in horse’s urine is presented in this paper. This method is based on the detection by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-high resolution mass spectrometry (GC-HRMS) of the trimethylsilyl (TMS) derivatives of the aglycon moieties of these compounds after solid phase extraction and methanolysis, according to the standard procedure currently used for the screening of anabolic steroids in horses, in the Athens Doping Control Laboratory. This validated method was also applied for the excretion study of digoxin in two mare horses after a single administration of a 75 μg Kg−1 oral dose, by GC-MS and GC-HRMS.

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Authors and Affiliations

  1. Doping Control Laboratory of Athens, Olympic Athletic Center of Athens, Kifissias 37, 15123, Maroussi, Greece

    P. Kiousi, Y. S. Angelis & C. G. Georgakopoulos

  2. National and Kapodistrian, University of Athens, Chemistry Department, Laboratory of Analytical Chemistry Panepistimiopolis 15771 Zographou, Athens, Greece

    M. Koupparis

  3. Department of Biochemistry and Biotechnology, University of Thessaly, Larisa, Greece

    D. Kouretas

  4. Faculty of Veterinary Medicine, Aristotelian University of Thessaloniki, Greece

    N. Diakakis & A. Desiris

Authors
  1. P. Kiousi
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  2. Y. S. Angelis
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  3. M. Koupparis
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  4. D. Kouretas
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  5. N. Diakakis
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  6. A. Desiris
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  7. C. G. Georgakopoulos
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Corresponding author

Correspondence to C. G. Georgakopoulos.

Additional information

In Honour of Professor Edward Houghton: A Celebration of 30 Years in Racing Chemistry

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Kiousi, P., Angelis, Y., Koupparis, M. et al. Gas Chromatographic – Mass Spectrometric Cardiotonic Glycosides Detection in Equine Urine Doping Analysis. Chromatographia 59 (Suppl 1), S105–S108 (2004). https://doi.org/10.1365/s10337-004-0214-z

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  • DOI: https://doi.org/10.1365/s10337-004-0214-z

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