Skip to main content
SpringerLink
Log in

Development of a Solid-Phase Extraction Procedure for the Simultaneous Determination of Polyphenols, Organic Acids and Sugars in Wine

  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A solid-phase extraction (SPE) procedure that fractionates wine samples into 2 sub-samples containing sugars and organic acids (sub-sample 1) and low molecular weight polyphenols (sub-sample 2), respectively, together with rugged LC procedures for their analyses are described. Wine is adjusted to pH 2.5 and loaded on a styrene-divinylbenzene (SDB) cartridge. The organic acids and sugars are eluted with 20 mM sulphuric acid and the monomeric polyphenols with ethyl acetate. Glucose and fructose are analysed by normal phase LC with evaporative light scattering detection and the organic acids by ion exclusion chromatography with UV detection at 210 nm. Analysis of the phenolic fraction is performed by reversed phase LC with diode array detection. Recoveries and repeatability’s for 27 standard compounds (2 sugars, 7 organic acids and 18 polyphenols) are presented. The method represents an improvement in terms of productivity and robustness compared to currently used procedures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ribereau-Gayon P, Gloris Y, Maujean A, Dubourdieu D (2000) Handbook of Enology – Volume 2, The Chemistry of Wine, Stabilization and Treatments, John Wiley & Sons, pp. 3–39, 55–80

  • Walsch B (1997) Tannin sensory perception and its relationship to other flavour contributors. In: Proceedings of the ASVO Oenology Seminar: Phenolics and Extraction, 3. M. Allen, G. Wall, N. Bullied (Eds), Australian Society of Viticulture and Oenology, pp. 24–27

  • Thorngate JH, Noble AC (1995) J. Sci. Food Agric. 67:531–535

    Google Scholar 

  • van Acker SABE, van der Vijgh WJV, Bast A (1998) Structural Aspects of Antioxidant Activity of Flavonoids. In: Flavonoids in Health and Disease, C. A. Rice-Evans, L. Packer (Eds), Marcel Dekker, Inc., New York, pp. 221–253

  • Terao J, Piskula K (1998) Flavonoids as Inhibitors of Lipid Peroxidation in Membranes. In: Flavonoids in Health and Disease, C. A. Rice-Evans, L. Packer (Eds), Marcel Dekker, Inc., New York, pp. 277–294

  • Badoud R, Pratz G (1986) J. Chromatogr. 360:119–136

    Google Scholar 

  • Marcé RM, Calull M, Olucha JC, Borrull F, Rius FX (1991) J Chromatogr 542:277–293

    Article  Google Scholar 

  • García Romero E, Sánchez Muñoz G, Martín Alvarez PJ, Ibáñez MD (1993) J Chromatogr. 360:111–117

  • Marcé RM, Calull M, Borrull F, Rius FX (1990) Am J Enol Vitic 41(4):289–294

    Google Scholar 

  • Arellano M, Andrianary J, Dedieu F, Couderc F, Puig Ph (1997) J Chromatogr 765:321–328

    Article  CAS  Google Scholar 

  • Kandl T, Kupina S (1999) Am J Enol Vitic 50(2):155–161

    CAS  Google Scholar 

  • Frayne RF (1986) Am J Enol Vitic 37(4):281–287

    CAS  Google Scholar 

  • Calull M, Marcé RM, Borrull F (1992) J Chromatogr 590:215–222

    Article  CAS  PubMed  Google Scholar 

  • Falqué López E, Fernández Gómez E (1996) J Chrom Science 34:254–257

    Google Scholar 

  • Klein H, Leubolt R (1993) J Chromatogr 640:259–270

    Article  CAS  Google Scholar 

  • Goreinstein S, Moshe R, Deutsch J, Wolfe FH, Tilis K, Stiller A, Flam I, Gat Ya (1991) J Food Compos Anal 5:236–245

    Google Scholar 

  • Clement A, Yong D, Brechet C (1992) J Liquid Chromatogr 15(5):805–817

    CAS  Google Scholar 

  • Engelhardt H, Ohs P (1987) Chromatographia 23(9):657–662

    CAS  Google Scholar 

  • Del Nozal MJ, Bernal JL, Gomez FJ, Antolin A, Toribio L (1992) J Chromatogr 607:191–198

    Article  Google Scholar 

  • Bernal JL, Del Nozal MJ, Toribio L, Del Alamo M (1996) J. Agric Food Chem. 44:507–511

    Google Scholar 

  • Rigaud J, Escribano-Bailon MT, Prieur C, Souquet JM, Cheynier V (1993) J Chromatogr A 654:255–260

    Article  CAS  Google Scholar 

  • Goldberg DM, Ng E, Karumanchiri A, Yan J, Diamandis EP, Soleas GJ (1995) J Chromatogr A 708:89–98

    Article  CAS  Google Scholar 

  • Rossi M, Di Tommaso D, Rotilio D (1998) Analysis of Wine Components by Capillary Electrophoresis. In: Proceedings of the 20th International Symposium on Capillary Chromatography (CD-ROM), Riva del Garda, Italy, P. Sandra, A. J. Rackstraw (Eds), No. H.21

  • Pazourek J, González G, Revilla AL, Havel J (2000) J Chromatogr A 874:111–119

    Article  CAS  PubMed  Google Scholar 

  • Vanhoenacker G, de Villiers A, Lazou K, De Keukeleire D, Sandra P (2001) Chromatographia 54(5-6)309–315

    Google Scholar 

  • Gu X, Chu Q, O’Dwyer M, Zeese M (2000) J Chromatogr A 881:471–481

    CAS  PubMed  Google Scholar 

  • Jaworski AW, Lee CY (1987) J Agric Food Chem 35:257–259

    CAS  Google Scholar 

  • Oszmianski J, Ramos T, Bourzeix M (1988) Am J Enol Vitic 39(3):259–262

    CAS  Google Scholar 

  • Oszmianski J, Sapis JC (1989) J Agric Food Chem 37:1293–1297

    CAS  Google Scholar 

  • Guillén DA, Barroso CG, Pérez-Bustamante JA (1996) J Chromatogr A 730:39–46

    Article  Google Scholar 

  • Guillén DA, Merello F, Barroso CG, Pérez-Bustamante JA (1997) J Agric Food Chem 45:403–406

    Google Scholar 

  • Goldberg DM, Karumanchiri A, Soleas GJ, Tsang E (1999) Am J Enol Vitic 50(2):185–193

    CAS  Google Scholar 

  • Cappiello A, Famiglini G, Mangani F, Careri M, Lombardi P, Mucchino C (1999) J Chromatogr A 855:515–527

    Article  CAS  PubMed  Google Scholar 

  • Pérez-Magariño AS, Revilla I, González-SanJosé ML, Beltrán S (1999) J Chromatogr A 847:75–81

    PubMed  Google Scholar 

  • Baptista JAB, da P. Tavares JF, Carvalho RCB (2001) Food Res International 34:345–355

    Article  CAS  Google Scholar 

  • Guillén DA, Barroso CG, Pérez-Bustamante JA (1996) J Chromatogr A 750:209–214

    Article  Google Scholar 

  • Gamoh K, Nakashima K (1999) Rapid Comm. Mass Spectrometry A 13:1112–1115

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. University of Stellenbosch, Department of Chemistry, CENSSUS, Private Bag X1, Matieland, 7602, South Africa

    A. de Villiers, F. Lynen, A. Crouch & P. Sandra

Authors
  1. A. de Villiers
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Lynen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Crouch
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Sandra
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. Sandra.

Rights and permissions

Reprints and permissions

About this article

Cite this article

de Villiers, A., Lynen, F., Crouch, A. et al. Development of a Solid-Phase Extraction Procedure for the Simultaneous Determination of Polyphenols, Organic Acids and Sugars in Wine. Chromatographia 59, 403–409 (2004). https://doi.org/10.1365/s10337-004-0204-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-004-0204-1

Keywords

Search

Navigation