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High-Performance Liquid Chromatographic Assay of Zonisamide (1,2-benzisoxazole-3-methanesulfonamide) in Human Plasma using a Solid-Phase Extraction Technique

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Abstract

A selective and sensitive liquid chromatographic method was developed for the determination of zonisamide in small volumes of plasma. Zonisamide and the internal standard methyl 4-hydroxybenzoate were extracted from 0.2 mL of plasma with solid-phase extraction columns and eluted with methanol. Analysis of the extracts was performed on a Symmetry C18 column with ultra-violet spectrophotometric detection. The calibration curve was linear over the concentration range of 0.05–5 μg mL−1 in plasma. Recoveries were reasonable for routine analyses; the limit of quantification was 0.05 μg mL−1 with a signal-to-noise ratio of 5. This method could be useful for the pharmacokinetic study of zonisamide in a limited volume of human plasma and for therapeutic drug monitoring.

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Authors and Affiliations

  1. Department of Pharmacology and Pharmacogenomics Research Center, Inje University College of Medicine and Clinical Pharmacology Center, Busan Paik Hospital, Busan, 614-735, Korea

    K.H. Liu, Y.K. Lee, Y.E. Sunwoo, W. Kang, S.S. Lee, Y.R. Yoon & J.G. Shin

  2. Department of Pharmacology and Clinical Pharmacology Unit, Seoul National University, College of Medicine and Hospital, Seoul, 110-799, Korea

    K.S. Yu

Authors
  1. K.H. Liu
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  2. Y.K. Lee
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  3. Y.E. Sunwoo
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  4. K.S. Yu
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  5. W. Kang
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  6. S.S. Lee
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  7. Y.R. Yoon
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  8. J.G. Shin
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Correspondence to J.G. Shin.

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Liu, K., Lee, Y., Sunwoo, Y. et al. High-Performance Liquid Chromatographic Assay of Zonisamide (1,2-benzisoxazole-3-methanesulfonamide) in Human Plasma using a Solid-Phase Extraction Technique. Chromatographia 59, 497–500 (2004). https://doi.org/10.1365/s10337-004-0194-z

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  • DOI: https://doi.org/10.1365/s10337-004-0194-z

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