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Comparison of Protein Precipitation, Turbulent Flow and Automated On-Line Solid Phase Extraction, as Plasma Sample Preparation Techniques for the Determination of Compound I by LC-MS-MS

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Abstract

Compound I is a novel subtype-selective 5-HT1 receptor antagonist currently under pre-clinical investigation. A theoretical increase in sensitivity of about 4 times over the plasma protein precipitation method by the two on-line extraction systems tested should be expected due to the introduction of 100% of the analyte onto the column with both the on-line systems. The comparison of these three methods showed that the real gain in sensitivity was 4 times with Prospekt2™ and 2 times with TurboFlow™. This might be explained by the different mobile phases used combined with different recoveries. Due to the improved chromatography, the higher sensitivity and lower carry-over observed for this compound, the Prospekt2™ system was chosen in order to validate an analytical procedure for the quantitation of I in rat plasma followed by LC-MS-MS.

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Authors and Affiliations

  1. Department of Pharmacokinetics, Dynamics & Metabolism, Pharmacia Italia S.p.A. – Pfizer Group, viale Pasteur 10, Nerviano, Milan, Italy

    S. Calderoli, E. Frigerio & C. A. James

Authors
  1. S. Calderoli
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  2. E. Frigerio
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  3. C. A. James
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Corresponding author

Correspondence to S. Calderoli.

Additional information

Presented at: 15th International Bioanalytical Forum. The Changing Role of Bioanalysis: Discovery to Market, Guildford, UK, July 1–4, 2003

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Calderoli, S., Frigerio, E. & James, C. Comparison of Protein Precipitation, Turbulent Flow and Automated On-Line Solid Phase Extraction, as Plasma Sample Preparation Techniques for the Determination of Compound I by LC-MS-MS. Chromatographia 59 (Suppl 2), S183–S186 (2004). https://doi.org/10.1365/s10337-003-0171-y

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  • DOI: https://doi.org/10.1365/s10337-003-0171-y

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