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A Comparison of the Retention Behaviour of Vitamin A and Some Metabolites Eluted from Four Different Reversed Phase High Performance Liquid Chromatographic Stationary Phases

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Abstract

Five retinoids, 13-cis-retinoic acid, 9-cis-retinoic acid, all-trans-retinoic acid, all-trans-retinol and 13-cis-retinal were isocratically separated from four different reversed phase high performance liquid chromatographic stationary phases. By taking advantage of the different retention mechanisms, present between the stationary phases and the analytes, the retinoids were separated with different elution orders using the same mobile phase composition. Two of the stationary phases appeared to have more possibilities to interact with the analytes than the usual hydrophobic interactions. The stationary phase with embedded polar groups showed hydrogen bonding properties and the calix[4]arene based stationary phase showed possibilities to form inclusion complexes with the analytes. These additional interactions appeared to benefit the separations of the analytes. This publication shows the benefits by isocratically separate retinoids employing other stationary phases than the conventional C18 stationary phase.

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Authors and Affiliations

  1. Institut für Organische Chemie, Universität Tübingen, Auf der Morgenstelle 18, 72076, Tübingen, Germany

    U. Skogsberg, D. Zeeb & K. Albert

Authors
  1. U. Skogsberg
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  2. D. Zeeb
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  3. K. Albert
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Correspondence to K. Albert.

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Skogsberg, U., Zeeb, D. & Albert, K. A Comparison of the Retention Behaviour of Vitamin A and Some Metabolites Eluted from Four Different Reversed Phase High Performance Liquid Chromatographic Stationary Phases. Chromatographia 59, 153–160 (2004). https://doi.org/10.1365/s10337-003-0168-6

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  • DOI: https://doi.org/10.1365/s10337-003-0168-6

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