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Determination of the Enantiomeric Purity of l-Carnitine and Acetyl-l-Carnitine by Chiral Liquid Chromatography

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Abstract

A chiral liquid chromatographic method for determination of the enantiomeric purity of both l-carnitine and acetyl-l-carnitine is described. Separation of the enantiomers of dl-carnitine and acetyl-dl-carnitine was achieved on a commercial chiral column (Chiralcel OD-R) after derivatization with (alpha-bromo)methyl phenyl ketone. Introduction of this lipophilic UV chromophoric group to the carnitine and acetylcarnitine molecules improved their retention, resolution, and UV detection. The mobile phase was 74:26 (v/v) 0.5 mol L-1 sodium perchlorate–acetonitrile, pH 3.8, and the flow rate was 0.4 mL min-1. Detection was performed at 235 nm. The method is selective and reliable for determination of the enantiomeric purity of bulk drug substances l-carnitine and acetyl-l-carnitine.

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Authors and Affiliations

  1. Department of Chemistry, School of Chemical Engineering and Materials Science, Beijing Institute of Technology, Beijing, 100081

    Meiling Qi

  2. Shenyang Pharmtech Institute of Pharmaceuticals, Shenyang, 110015, China

    Peng Wang & Yujing Sun

  3. Hebei Hengli Pharmaceutical Group, Xingtai, 054000, China

    Jianjun Yang

Authors
  1. Meiling Qi
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  2. Peng Wang
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  3. Yujing Sun
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  4. Jianjun Yang
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Correspondence to Meiling Qi.

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Qi, M., Wang, P., Sun, Y. et al. Determination of the Enantiomeric Purity of l-Carnitine and Acetyl-l-Carnitine by Chiral Liquid Chromatography. Chromatographia 59, 247–250 (2004). https://doi.org/10.1365/s10337-003-0151-2

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  • DOI: https://doi.org/10.1365/s10337-003-0151-2

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