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Investigation of Tomato Plant Defence Response to Tobacco Mosaic Virus by Determination of Methyl Salicylate with SPME-Capillary GC-MS

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Abstract

Headspace SPME was applied to investigation of tomato plant defence response to tobacco mosaic virus (TMV) by determination of compounds emitted from tomato plants at the extraction conditions of 25 °C and 15 min. It was found that TMV-inoculated tomato plant released large amount of methyl salicylate (MeSA) as response to TMV and MeSA concentration changed dramatically with time after inoculation. Gaseous MeSA as a signaling compound could induce in surrounding tomato plants to produce salicylic acid (SA) and synthesize and release MeSA. These results show that MeSA might be an airborne plant-signalling molecule in tomato plant response to TMV. The present method provided low detection limit of 2.0 ng L−1 and needed little sample preparation time (15 min), so the method makes it easy to find the critical times of tomato plant response to TMV by fast determination of MeSA released from tomato plant.

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Authors and Affiliations

  1. Department of Chemistry, Fudan University, Shanghai, 200433, China

    Chunhui Deng & Xiangmin Zhang

  2. Academy of Agriculture Science, Shanghai, 201106, China

    Weimin Zhu

  3. College of Life Science, Fudan University, Shanghai, 200433, China

    Ji Qian

Authors
  1. Chunhui Deng
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  2. Xiangmin Zhang
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  3. Weimin Zhu
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  4. Ji Qian
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Correspondence to Xiangmin Zhang.

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Deng, C., Zhang, X., Zhu, W. et al. Investigation of Tomato Plant Defence Response to Tobacco Mosaic Virus by Determination of Methyl Salicylate with SPME-Capillary GC-MS. Chromatographia 59, 263–268 (2004). https://doi.org/10.1365/s10337-003-0144-1

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  • DOI: https://doi.org/10.1365/s10337-003-0144-1

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