Abstract
A variety of compounds containing an amine group (i.e., amino acids and amino alcohols) are fast and much better than baseline enantioresolved on a teicoplanin bonded chiral phase using the methanol-based mobile phase after their pre-column derivatization with 2,4-dinitrofluorobenzene (DNFB) in alkaline medium. The resolution is found relatively insensitive to the structural variations of the analyte and is enhanced as the stereogenic center of analyte is close to the nitro group substituted aromatic moiety of the tagging reagent for stronger π-π interaction. The resolution is either not observed or unsatisfactory using the acetonitrile-based mobile phase.
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Shinbo T, Yamaguchi T, Nishimura K, Suguira M (1987) J Chromatogr 405:145–513
Hilton M, Armstrong DW (1991) J Liq Chromatogr 14:9–28
Hilton M, Armstrong DW (1991) J Liq Chromatogr 14:3673–3683
Gibitz G, Juffman E, Jelleng W (1982) Chromatographia 16:103–106
Davankov VA, Semechkin AV (1977) J Chromatogr 141:313–353
Armstrong DW, Yang X, Han SM, Menges RA (1987) Anal Chem 59:2594–2596
Sanger F (1945) Biochem J 45:563–567
Zukowski J, Pawlowska M, Armstrong DW (1992) J Chromatogr 623:33–41
Zukowski J, Pawlowska M, Nagatkina M, Armstrong DW (1992) J Chromatogr 629:169–179
Chang SC, Wang LR, Armstrong DW (1992) J Liq Chromatogr 15:1411–1429
Pawlowska M, Chen S, Armstrong DW (1993) J Chromatogr 641:257–265
Chen S (1996) J Chin Chem Soc 43:45–51
Chen S (1996) J Chin Chem Soc 43:503–506
Chen S (1999) J Chin Chem Soc 4:239–244
Chen S (1997) Chin Pharm J 43:51–60
Chen S (2003) Amino Acids (accepted)
Chen S (2003) J Liq Chromatogr (accepted)
Berthod A, Liu Y, Bagwill C, Armstrong DW (1996) J Chromatogr A 731:123–137
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Chen, S. The HPLC Stereoselective Resolution of N-2,4-Dinitrophenylated (DNP) Amine-Containing Enantiomers on Teicoplanin Bonded Phase Using the Methanol-Based Mobile Phase. Chromatographia 59, 173–180 (2004). https://doi.org/10.1365/s10337-003-0126-3
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DOI: https://doi.org/10.1365/s10337-003-0126-3