Summary
A new, rapid and simple method is described and applied to resolve and quantify mixtures of prednisolone, Zn-bacitracin and phenylephrine. The determination was accomplished by MEKC. The separation was carried out at 25 °C and 30 kV, using a 5 mM phosphate-5 mM borate buffer (pH=8.2), 40 mM SDS as background electrolyte. Under these conditions, the run time was 6.6 min and the limits of quantification were about 1.0 mg L−1 for every component. Repeatability and reproducibility studies were achieved showing no significant differences at 95% confidence level. The MEKC method has been applied for quantifying these compounds in different commercials pharmaceuticals products, without separations steps.
Similar content being viewed by others
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lemus Gallego, J., Arroyo, J. Optimized Method for the Determination of Prednisolone, Zn-Bacitracin and Phenylephrine in Local Pharmaceutical Preparations by Micellar Electrokinetic Capillary Chromatography. Chromatographia 58, 277–281 (2003). https://doi.org/10.1365/s10337-003-0014-x
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1365/s10337-003-0014-x