Chromatographia

, Volume 72, Issue 11–12, pp 1089–1095 | Cite as

Simultaneous Determination of Avermectin and Milbemycin Residues in Bovine Tissue by Pressurized Solvent Extraction and LC with Fluorescence Detection

  • Xi Xia
  • Zhiming Xiao
  • Qiushi Huang
  • Lijun Xia
  • Kui Zhu
  • Xiaolin Wang
  • Jianzhong Shen
  • Shuangyang Ding
Original
First Online:
Received:
Revised:
Accepted:

Abstract

A rapid and sensitive method has been developed for the simultaneous determination of four avermectins and one milbemycin residues in bovine tissue. The isolation of the analytes from muscle and liver samples was accomplished utilizing a pressurized solvent extractor. The optimized extraction procedure using acetonitrile/water (40:60, v/v) as extraction solvent, was automatically carried out at 100 °C and 10 MPa, applying two static cycles for 3 min. The extracts were cleaned up on a C18 solid-phase extraction cartridge and analyzed by liquid chromatography with fluorescence detection after derivatization. Mean recoveries of the five analytes from fortified samples were between 84.8 and 101.8%, with relative standard deviations lower than 10.8%. The limit of detection and quantification were in the ranges of 0.1–0.2 and 0.5–0.6 μg kg–1, respectively. The application of the newly developed method was demonstrated by analyzing bovine meat samples from market.

Keywords

Column liquid chromatography–fluorescence detection Pressurized solvent extraction Bovine tissue Avermectins and milbemycins 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

This study was supported by the China National Science and Technology Pillar Program in the Eleventh 5-year plan period (2009BADB7B05) and The program for Cheung Kong Scholars and Innovative Research Team in University of China (No. IRT0866). The authors also appreciate Prof. Ming Wang (China Agricultural University, Beijing, China) for the generous gift of eprinomectin standard.

References

  1. 1.
    Campbell WC, Fisher MH, Stapley EO (1983) Science 221:823–828CrossRefGoogle Scholar
  2. 2.
    Forbes AB (1993) Vet Parasitol 48:19–28. doi: 10.1016/0304-4017(93)90141-9 CrossRefGoogle Scholar
  3. 3.
    Shoop WL, Mrozik H, Fisher MH (1995) Vet Parasitol 59:139–156. doi: 10.1016/0304-4017(94)00743-V CrossRefGoogle Scholar
  4. 4.
    Sheridan R, Desjardins L (2006) J AOAC Int 89:1088–1094Google Scholar
  5. 5.
    Durden DA (2007) J Chromatogr B 850:134–146. doi: 10.1016/j.jchromb.2006.11.014 CrossRefGoogle Scholar
  6. 6.
    Ali MS, Sun T, McLeroy GE, Phillippo ET (2000) J AOAC Int 83:31–38Google Scholar
  7. 7.
    Ministry of Agriculture of People’s Republic of China, 235th bulletin (2002)Google Scholar
  8. 8.
    European Agency for the Evaluation of Veterinary Medicines (1998) EMEA/MRL/520/98-FINALGoogle Scholar
  9. 9.
    Danaher M, Howells LC, Crooks SRH, Cerkvenik-Flajs V, O’Keeffe M (2006) J Chromatogr B 844:175–203. doi: 10.1016/j.jchromb.2006.07.035 CrossRefGoogle Scholar
  10. 10.
    Jiang H, Zhou D, Li H, Xu F, Li C, Shen J, Li X, Ding S (2008) Chromatographia 68:259–262. doi: 10.1365/s10337-008-0688-1 CrossRefGoogle Scholar
  11. 11.
    Yoshii K, Kaihara A, Tsumura Y, Ishimitsu S, Tonogai Y (2001) J AOAC Int 84:910–917Google Scholar
  12. 12.
    Nagata T, Miyamoto F, Hasegawa Y, Ashizawa E (2003) J AOAC Int 86:490–493Google Scholar
  13. 13.
    Hou X, Wu Y, Shen J, Wang L, Ding S (2007) Chromatographia 65:77–80. doi: 10.1365/s10337-006-0098-1 CrossRefGoogle Scholar
  14. 14.
    Walker DK, Fenner KS (2000) J Pharm Biomed Anal 24:105–111. doi: 10.1016/S0731-7085(00)00415-5 CrossRefGoogle Scholar
  15. 15.
    Chen YC, Hung YP, Fleckenstein L (2002) J Pharm Biomed Anal 29:917–926. doi: 10.1016/S0731-7085(02)00120-6 CrossRefGoogle Scholar
  16. 16.
    Turnipseed SB, Roybal JE, Andersen WC, Kuck LR (2005) Anal Chim Acta 529:159–165. doi: 10.1016/j.aca.2004.07.061 CrossRefGoogle Scholar
  17. 17.
    Hou XL, Jiang HY, Ding SY, Zhang SX, Li XW, Shen JZ (2006) J AOAC Int 89:1110–1115Google Scholar
  18. 18.
    Danaher M, O’Keeffe M, Glennon JD (2001) J Chromatogr B 761:115–123. doi: 10.1016/S0378-4347(01)00315-2 CrossRefGoogle Scholar
  19. 19.
    Wu ZR, Li JS, Zhu LL, Luo HP, Xu XJ (2001) J Chromatogr B 755:361–366. doi: 10.1016/S0378-4347(01)00077-9 CrossRefGoogle Scholar
  20. 20.
    He JH, Hou XL, Shen JZ, Jiang HY (2005) J AOAC Int 88:1099–1103Google Scholar
  21. 21.
    Hou X, Li X, Ding S, He J, Jiang H, Shen J (2006) Chromatographia 63:543–550. doi: 10.1365/s10337-006-0790-1 CrossRefGoogle Scholar
  22. 22.
    Carabias-Martínez R, Rodríguez-Gonzalo E, Revilla-Ruiz P, Hernández-Méndez J (2005) J Chromatogr A 1089:1–17. doi: 10.1016/j.chroma.2005.06.072 CrossRefGoogle Scholar
  23. 23.
    Shao B, Han H, Li D, Ma Y, Tu X, Wu Y (2007) Food Chem 105:1236–1241. doi: 10.1016/j.foodchem.2007.02.040 CrossRefGoogle Scholar
  24. 24.
    Reid AM, Brougham CA, Fogarty AM, Roche JJ (2009) Anal Chim Acta 634:197–204. doi: 10.1016/j.aca.2008.12.032 CrossRefGoogle Scholar
  25. 25.
    Blasco C, Corcia AD, Picó Y (2009) Food Chem 116:1005–1012. doi: 10.1016/j.foodchem.2009.03.055 CrossRefGoogle Scholar

Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010

Authors and Affiliations

  • Xi Xia
    • 1
  • Zhiming Xiao
    • 1
  • Qiushi Huang
    • 1
  • Lijun Xia
    • 1
  • Kui Zhu
    • 1
  • Xiaolin Wang
    • 1
  • Jianzhong Shen
    • 1
  • Shuangyang Ding
    • 1
  1. 1.Department of Pharmacology and Toxicology, College of Veterinary MedicineChina Agricultural UniversityBeijingPeoples’ Republic of China

Personalised recommendations