, Volume 72, Issue 7–8, pp 737–741 | Cite as

Determination of Cinepazide Maleate in Rat Plasma by LLE Using LC–MS–MS

  • Guanyang Lin
  • Jiayin Zhu
  • Lufeng Hu
  • Xuebao Wang
  • Xuezhi Yang
  • Xianqin WangEmail author
Full Short Communication


A liquid chromatography tandem mass spectrometry method employing electrospray ionization has been developed for the determination of cinepazide maleate in rat plasma using alprazolam as the internal standard. Liquid–liquid extraction was used for sample preparation and the analysis was achieved on a C18 reversed phase column. The method was validated over the concentration range 1–2,000 ng mL−1 in rat plasma. The intra-day and inter-day precision of cinepazide maleate expressed as RSD < 10% and the relative error (RE) did not exceed 10%. This validated method is a novel technique for sample preparation and quantitation, which was successfully applied to pharmacokinetic studies of cinepazide maleate in rats following a single oral administration dose of 10 mg kg−1.


Column liquid chromotography–tandem mass spectrometry Pharmacokinetic study Cinepazide maleate Plasma 


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Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010

Authors and Affiliations

  • Guanyang Lin
    • 1
  • Jiayin Zhu
    • 2
  • Lufeng Hu
    • 1
  • Xuebao Wang
    • 3
  • Xuezhi Yang
    • 1
  • Xianqin Wang
    • 3
    Email author
  1. 1.The First Affiliated Hospital of Wenzhou Medical CollegeWenzhouChina
  2. 2.Laboratory Animal CenterWenzhou Medical CollegeWenzhouChina
  3. 3.Analytical and Testing CenterWenzhou Medical CollegeWenzhouChina

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