Chromatographia

, Volume 71, Issue 11–12, pp 1081–1086 | Cite as

Rapid RP-LC Method with Fluorescence Detection for Analysis of Fexofenadine in Human Plasma

  • Lucyna Konieczna
  • Alina Plenis
  • Ilona Olędzka
  • Piotr Kowalski
  • Tomasz Bączek
Full Short Communication

Abstract

A rapid and sensitive reversed-phase high-performance liquid chromatographic method for analysis of fexofenadine in human plasma has been developed and optimized. The analytes were extracted from biological samples by solid-phase extraction on hydrophilic–lipophilic balance cartridges. LC separation was performed on a C18 analytical column (125 mm × 4 mm i.d., 5-μm particles) with 42:58 (v/v) acetonitrile–water adjusted to pH 2.7 with 85% orthophosphoric acid as mobile phase. Fluorescence detection was performed with excitation at 230 nm and emission at 290 nm. The total time for chromatographic separation was 7 min. The method was validated in accordance with EU guidelines by analysis of plasma samples fortified with fexofenadine at concentrations between 0.05 and 800 ng mL−1. Calibration plots were linear in this range. Mean recovery was typically 94.03% and the detection limit was 0.05 ng mL−1. The time required for quantitative analysis is shorter than that required by other methods.

Keywords

Column liquid chromatography Fluorescence detection Solid-phase extraction Fexofenadine 

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Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010

Authors and Affiliations

  • Lucyna Konieczna
    • 1
  • Alina Plenis
    • 1
  • Ilona Olędzka
    • 1
  • Piotr Kowalski
    • 1
  • Tomasz Bączek
    • 1
  1. 1.Department of Pharmaceutical ChemistryMedical University of GdańskGdańskPoland

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