Chromatographia

, Volume 71, Issue 5–6, pp 383–388 | Cite as

Simultaneous Determination of Fluoroquinolones, Tetracyclines and Sulfonamides in Chicken Muscle by UPLC–MS–MS

  • Jianzhong Shen
  • Liming Guo
  • Fei Xu
  • Qinxiong Rao
  • Xi Xia
  • Xiaowei Li
  • Shuangyang Ding
Original
First Online:
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Revised:
Accepted:

Abstract

A rapid and sensitive analytical method for the simultaneous determination of four fluoroquinolones, four tetracyclines and six sulfonamides in chicken muscle using ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC–MS–MS) has been developed and validated. Samples were extracted with McIlvaine buffer-acetonitrile, defatted with n-hexane, and analyzed by UPLC–MS–MS. Solvent delay technique was applied in the analysis to remove the non-volatile phosphate and carry out farther on-line SPE clean-up. Satisfactory recoveries (55–110%) of all the veterinary drugs were demonstrated in 1, 10 and 20 μg kg−1 spiked levels with the overall RSD for intra- and inter-day of 14 analytes less than 18%. The LOD and LOQ were 0.3 and 1.0 μg kg−1, respectively. Quantitative results of 103 real samples indicated that the present method was suitable for the quantitative analysis of real samples.

Keywords

Ultra-performance liquid chromatography–tandem mass spectrometry Veterinary drugs Fluoroquinolones, tetracyclines, and sulphonamides in chicken muscle 
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Notes

Acknowledgments

This work was supported by the National Basic Science Research for the 11th Five-Year plan (No.2006BAD31B09-2). The authors appreciate the cooperation of other faculty members in the Department of Pharmacology and Toxicology of the Veterinary Medicine at the China Agriculture University.

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2010

Authors and Affiliations

  • Jianzhong Shen
    • 1
  • Liming Guo
    • 1
  • Fei Xu
    • 1
  • Qinxiong Rao
    • 2
  • Xi Xia
    • 1
  • Xiaowei Li
    • 1
  • Shuangyang Ding
    • 1
  1. 1.National Reference Laboratories for Veterinary Drug Residue, College of Veterinary MedicineChina Agricultural UniversityBeijingPeople’s Republic of China
  2. 2.Institute for Agri-Food Standards and TestingShanghai Academy of Agricultural SciencesShanghaiPeople’s Republic of China

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