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Fast Analysis of Statins in Pharmaceuticals by MEKC

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Abstract

A universal micellar electrokinetic capillary chromatographic (MEKC) method with diode-array detection for the simultaneous and short-time analysis of lovastatin, simvastatin, pravastatin, fluvastatin, atorvastatin and rosuvastatin was introduced. Base hydrolysis was used to open lactone ring of lovastatin and simvastatin, administered as lactone prodrugs, in order to transform these compounds to the corresponding β-hydroxyl acid forms before MEKC analysis. This approach offered shorter analysis time due to a decrease of the migration times of negatively charged statin drugs in comparison to neutral lactone forms. Optimized conditions were found to be a 25 mM borate buffer pH 9.5 with 25 mM sodium dodecyl sulphate and 10% methanol added as an organic modifier, an applied voltage of 23 kV and a separation temperature of 30 °C. Ketoprofen was used as an internal standard. The linearity of the detector response for each statin was within the concentration range from 10 to 100 μg mL−1 with a correlation coefficient greater than 0.9994. Analyses of six statin drugs in pharmaceutical samples were carried out in only 5 min. The interference of the tablet sample matrix was not observed. The recovery values were in the range of 98.04–100.80%.

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References

  1. Kaufmann DW, Kelly JP, Rosenberg L, Anderson TE, Michell AA (2002) J Am Med Assoc 287:337–344

    Article  Google Scholar 

  2. Balk EM, Karas RH, Jordan HS, Kupelnick B, Chew P, Lau J (2004) Am J Med 117:775–790

    Article  CAS  Google Scholar 

  3. Mason P (2004) Intensive Crit Care Nurs 20:53–55

    Article  CAS  Google Scholar 

  4. Schachter M (2004) Fundam Clin Pharmacol 19:117–125

    Article  Google Scholar 

  5. McTaggart F (2003) Atheroscler Suppl 4:9–14

    Article  CAS  Google Scholar 

  6. Corsini A, Maggi FM, Catapano AL (1995) Pharmacol Res 34:9–27

    Article  Google Scholar 

  7. Ertürk S, Önal A, Çetin SM (2003) J Chromatogr B 793:193–205

    Article  Google Scholar 

  8. Nirogi R, Mudigonda K, Kandikere V (2007) J Pharm Biomed Anal 44:379–387

    Article  CAS  Google Scholar 

  9. Nováková L, Šatínský D, Solich P (2008) Trends Analyt Chem 27:352–367

    Article  Google Scholar 

  10. Miao XS, Metcalfe CD (2003) J Chromatogr A 998:133–141

    Article  CAS  Google Scholar 

  11. Pasha MK, Muzeeb S, Basha SJS, Shashikumar D, Mullangi R, Srinivas NR (2006) Biomed Chromatogr 20:282–293

    Article  CAS  Google Scholar 

  12. Nováková L, Arnal Lopéz S, Solichová D, Šatínský D, Kulichová B, Horna A, Solich P (2009) Talanta 78:834–839

    Article  Google Scholar 

  13. Altria KD, Marsh A, Sanger-van de Griend C (2006) Electrophoresis 27:2263–2282

    Article  CAS  Google Scholar 

  14. Terabe S (2004) Anal Chem 76:240A–246A

    Article  CAS  Google Scholar 

  15. Injac R, Kočevar N, Kreft S (2007) Anal Chim Acta 594:119–127

    Article  CAS  Google Scholar 

  16. Görög S (2008) J Pharm Biomed Anal 48:247–253

    Article  Google Scholar 

  17. Suntornsuk L (2007) J Chromatogr Sci 45:559–577

    CAS  Google Scholar 

  18. Kocijan A, Grahek R, Bastrada A, Zupancic-Kralj L (2005) J Chromatogr B 822:311–315

    Article  CAS  Google Scholar 

  19. Nigović B, Vegar I (2008) Croat Chem Acta 81:615–622

    Google Scholar 

  20. Nigović B, Damić M, Injac R, Kočevar Glavač N, Štrukelj B (2009) Chromatographia 69:1299–1305

    Article  Google Scholar 

  21. Guihen E, Sisk GD, Scully NM, Glennon JD (2006) Electrophoresis 27:2338–2347

    Article  CAS  Google Scholar 

  22. Süslü İ, Çelebier M, Altinöz S (2007) Chromatographia 66:S65–S72

    Article  Google Scholar 

  23. Kittell J, Borup B, Voladari R, Zahn K (2005) Metab Eng 7:53–58

    Article  CAS  Google Scholar 

  24. Li M, Fan LY, Zhang W, Cao CX (2007) Anal Bioanal Chem 387:2719–2725

    Article  CAS  Google Scholar 

  25. Javernik Rajh S, Kreft S, Štrukelj B, Vrečer F (2003) Croat Chem Acta 76:263–268

    Google Scholar 

  26. Srinivasu MK, Raju AN, Reddy GO (2002) J Pharm Biomed Anal 29:715–721

    Article  CAS  Google Scholar 

  27. Trung TQ, Dung PT, Hoan NN, Kim DJ, Lee JH, Kim KH (2008) Arch Pharm Res 31:1066–1072

    Article  CAS  Google Scholar 

  28. European Pharmacopoeia, 6th edn. (2007) Council of Europe, Strasbourg

  29. Ishihama J, Nakamura M, Miwa T, Kajima T, Asakawa N (2002) J Pharm Sci 91:933–942

    Article  CAS  Google Scholar 

  30. Miller JM, Blackburn AC, Shi Y, Melzak AJ, Ando HY (2002) Electrophoresis 23:2833–2841

    Article  CAS  Google Scholar 

  31. McTavish D, Sorkin EM (1991) Drugs 42:65–89

    Article  CAS  Google Scholar 

  32. McTaggart F, Buckett L, Davidson R (2001) Am J Cardiol 87(Suppl B):28–32

    Article  Google Scholar 

  33. Mayer BX (2001) J Chromatogr A 907:21–37

    Article  CAS  Google Scholar 

  34. International Conference on Harmonization (ICH) (2005) Validation of analytical procedures: text and methodology Q2 (R1)

  35. Injac R, Bošković M, Kočevar N, Vovk T (2008) Anal Chim Acta 620:150–161

    Article  CAS  Google Scholar 

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Acknowledgement

This work was supported through Grant No. 0061117-1240 (Investigation of new methods in analysis of drugs and bioactive substances) from the Ministry of Science, Education and Sports of the Republic of Croatia.

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Authors and Affiliations

  1. Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000, Zagreb, Croatia

    Miranda Damić & Biljana Nigović

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  1. Miranda Damić
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  2. Biljana Nigović
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Correspondence to Biljana Nigović.

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Damić, M., Nigović, B. Fast Analysis of Statins in Pharmaceuticals by MEKC. Chroma 71, 233–240 (2010). https://doi.org/10.1365/s10337-009-1432-1

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