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An In Vitro Study of the Interaction of Copper(II) Salicylates with Genomic DNA Using Gel Electrophoresis and Gel Permeation Chromatography

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Abstract

This papers describes the in vitro interaction of copper(II) acetylsalicylate and copper(II) salicylate with genomic DNA isolated from human blood. The two drug substances were found to bind to DNA after incubation with whole blood over night. Bonding was confirmed by detection of separated DNA electrophoresis bands for copper, copper(II) acetylsalicylate, copper(II) salicylate, acetylsalicylic acid and salicylic acid. Drug–DNA interactions were observed during electrophoresis in the form of fragmentation by formation of two bands when compared to controls. Gel permeation chromatography parameters also confirmed the occurrence of fragmentation. The use of gel permeation chromatography parameters as a measure of fragmentation of DNA is discussed. The fragmentation of genomic DNA after incubation with copper(II) acetylsalicylate and copper(II) salicylate suggested that these drug substances might be responsible for cytotoxicity in vivo.

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References

  1. Graham DY, Smith JL (1986) Ann Intern Med 104:390–398

    CAS  Google Scholar 

  2. Roderick PJ, Wilkes HC, Meade TW (1993) Br J Clin Pharmacol 35:219–226

    CAS  Google Scholar 

  3. Sorenson JRJ (1976) J Med Chem 19:135–148. doi:10.1021/jm00223a024

    Article  CAS  Google Scholar 

  4. Chohan ZH, Iqbal MS, Iqbal HS, Scozzafava A, Supuran CT (2002) J Enz Inhib Med Chem 17:87–91. doi:10.1080/14756360290030734

    Article  CAS  Google Scholar 

  5. Kishore V (1988) Res Commun Chem Pathol Pharmacol 60:257–260

    CAS  Google Scholar 

  6. Lewis J (1978) Inflam Res 8:244–250

    CAS  Google Scholar 

  7. Sorenson JRJ (1978) Prog Med Chem 15:211–260. doi:10.1016/S0079-6468(08)70257-1

    Article  CAS  Google Scholar 

  8. Anonymous (1974) JAMA 229:1268–1269

    Google Scholar 

  9. Malkiel S, Har-el R, Schwalb H, Uretzky G, Borman JB, Chenion M (1993) Free Rad Res Commun 18:7–15. doi:10.1007/BF02602957

    Article  CAS  Google Scholar 

  10. Mazzetti I, Grigolo B, Borzi RM, Meliconi R, Facchini A (1996) Int J Clin Lab Res 26:245–249

    Article  CAS  Google Scholar 

  11. Tyrer LB (1984) J Reprod Med 29:547–550

    CAS  Google Scholar 

  12. Sorenson JRJ (1982) Inflammatory diseases and copper. Humana, Clifton

    Google Scholar 

  13. Iqbal MS, Sher M, Pervez H, Saeed M (2008) Biol Trace Elem Res 124:283–288. doi:10.1007/s12011-008-8146-3

    Article  CAS  Google Scholar 

  14. Drouin R, Rodriguez H, Gao TS-W, Gebreyes Z, O’connor TR, Holmquist GP, Akman SA (1996) Free Rad Biol Med 21:261–273. doi:10.1016/0891-5849(96)00037-8

    Article  CAS  Google Scholar 

  15. Alich AA, Wittmers LE (1980) J Pharm Sci 69:725–727. doi:10.1002/jps.2600690631

    Article  CAS  Google Scholar 

  16. Borer LL, Barry E (2000) J Chem Edu 77:354–356

    Article  CAS  Google Scholar 

  17. Sorenson JRJ, Oberley LW (1990) US Pat 4952607

Download references

Acknowledgment

The authors are grateful to the GC University for a financial aid from its research fund.

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Authors and Affiliations

  1. Department of Chemistry, GC University, Lahore, 54000, Pakistan

    Mohammad S. Iqbal, Raazia Arif & Sadia Fatima

  2. Department of Zoology, GC University, Lahore, 54000, Pakistan

    Mohammad S. Mughal

  3. Department of Pharmacy, University of Sargodha, Sargodha, Pakistan

    Muhammad Sher

Authors
  1. Mohammad S. Iqbal
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  2. Mohammad S. Mughal
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  3. Raazia Arif
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  4. Sadia Fatima
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  5. Muhammad Sher
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Correspondence to Mohammad S. Iqbal.

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Iqbal, M.S., Mughal, M.S., Arif, R. et al. An In Vitro Study of the Interaction of Copper(II) Salicylates with Genomic DNA Using Gel Electrophoresis and Gel Permeation Chromatography. Chroma 70, 1659–1663 (2009). https://doi.org/10.1365/s10337-009-1404-5

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  • DOI: https://doi.org/10.1365/s10337-009-1404-5

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