Chromatographia

, Volume 71, Issue 1–2, pp 125–129 | Cite as

A Validated LC Method for Simultaneous Determination of Three Major Components in Entada phaseoloides

  • Yuqiong Dong
  • Haiming Shi
  • Mengyue Wang
  • Xiaobo Li
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Abstract

A high performance liquid chromatography method was developed for the simultaneous determination of three major active constituents in Entada phaseoloides, namely phaseoloidin (1), entadamide A (2), entadamide A-β-d-glucopyranoside (3), respectively. The samples were separated on an Aglient Eclipse XDB-C18 column with gradient elution of acetonitrile and 0.3% phosphoric acid (v/v) at a flow rate of 1.0 mL min−1 and detected at 280 nm. The three target compounds were completely separated within 10 min. All calibration curves showed good linearity (r > 0.9999) within test ranges. The reproducibility was evaluated by intra- and inter-day assays and RSD values were less than 1.04%. The recoveries were between 97.15 and 101.95%. The method was successfully applied to the analysis of three compounds in 22 commercial samples of E. phaseoloides. The results indicated that the developed LC assay was readily utilized as a quality control method for E. phaseoloides.

Keywords

Column liquid chromatography Phaseoloidin Entadamide A Entadamide A-β-d-glucopyranoside Entada phaseoloides 
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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Yuqiong Dong
    • 1
  • Haiming Shi
    • 1
  • Mengyue Wang
    • 1
  • Xiaobo Li
    • 1
  1. 1.School of PharmacyShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China

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