Chromatographia

, 70:1207 | Cite as

LC Analysis of Histamine and Other Biogenic Amines in Digesta Cultures

  • Wiebke Engelmann
  • Annette Zeyner
  • Klaus D. Markuske
  • Jörg R. Aschenbach
Full Short Communication
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Abstract

In the intestine, microbial protein degradation to histamine and other biologically active amines is believed to be relevant to the health of species. An LC method with fluorescence detection has been developed for analysis of histamine, phenylethylamine, isoamylamine, putrescine, cadaverine, tyramine, and spermidine. After pre-column derivatisation with 9-fluorenylmethyl chloroformate, amines were separated on a reversed-phase C18 polyamine column with a mobile phase gradient. Validation of the method included calibration experiments, determination of amine recovery, and repeatability tests. The method proved especially useful for detection of histamine, phenylethylamine, putrescine, and cadaverine at relevant concentrations in caecum cultures from horses. The limits of quantification for these four amines ranged between 80 nM (histamine) and 400 nM (phenylethylamine).

Keywords

Column liquid chromatography Biogenic amines Caecum simulation technique Digesta cultures Histamine 
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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Wiebke Engelmann
    • 1
  • Annette Zeyner
    • 1
    • 2
  • Klaus D. Markuske
    • 1
  • Jörg R. Aschenbach
    • 3
  1. 1.Institute of Animal Nutrition, Nutritional Diseases and DieteticsUniversity of LeipzigLeipzigGermany
  2. 2.Faculty of Agricultural and Environmental Sciences, Institute of Farm Animal Sciences and TechnologyUniversity of RostockRostockGermany
  3. 3.Institute for Veterinary PhysiologyUniversity of LeipzigLeipzigGermany

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