Chromatographia

, 70:1191 | Cite as

Isolation of Terpenoids from Trichilia quadrijuga (Meliaceae) by Droplet Counter-Current Chromatography

  • Virginia Freitas Rodrigues
  • Hadria M. Carmo
  • Rodrigo R. Oliveira
  • Raimundo Braz Filho
  • Leda Mathias
  • Ivo José C. Vieira
Full Short Communication
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Abstract

The Trichilia genus (Meliaceae) consists of about 230 species distributed throughout tropical America and its phytochemical profile is rich in terpenic metabolites. Droplet counter-current chromatography (DCCC) was used for the isolation and purification of secondary metabolites obtained from a dichloromethane fraction (2.9 g) of Trichilia quadrijuga stems. A hexane–ethyl acetate–methanol–water (1:2:1.75:1, v/v/v/v) solvent system was chosen to isolate 2β,3β,4β-trihydroxypregnan-16-one (1, 24.5 mg, 0.8%), kudtdiol (2, 45.0 mg, 1.6%) and 3-O-β-d-glucopyranosylsitosterol (3, 6.0 mg, 0.2%). The results showed that DCCC was a very effective tool for the isolation of terpenes from T. quadrijuga.

Keywords

Droplet counter-current chromatography Terpenoids Meliaceae Trichilia quadrijuga 
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Notes

Acknowledgments

The authors are grateful to the technician Eliane Gonçalves de Carvalho from the Centro Analítico of Far-Manguinhos (Fiocruz) for the aqcisition of NMR spectra, the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ).

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Virginia Freitas Rodrigues
    • 1
    • 2
  • Hadria M. Carmo
    • 2
  • Rodrigo R. Oliveira
    • 2
  • Raimundo Braz Filho
    • 2
  • Leda Mathias
    • 2
  • Ivo José C. Vieira
    • 1
    • 2
  1. 1.Laboratório de Tecnologia de AlimentosUniversidade Estadual do Norte Fluminense Darcy RibeiroCampos dos GoytacazesBrazil
  2. 2.Laboratório de Ciências QuímicasUniversidade Estadual do Norte Fluminense Darcy RibeiroCampos dos GoytacazesBrazil

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