Determination of Histamine in Rat Plasma and Tissue Extracts by Intramolecular Excimer-Forming Derivatization and LC with Fluorescence Detection
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A highly sensitive and selective method for determination of histamine in rat plasma and tissue extracts by liquid chromatography with fluorescence detection is described. The method is based on precolumn derivatization of amino groups of histamine with two molecules of 4-(1-pyrene)butyric acid N-hydroxysuccinimide ester that allows generation of intramolecular excimer fluorescence. The detection limit for histamine was 0.183 nM. This sensitivity allowed determination of histamine in 10 μL of rat plasma or in the extracts from less than 1 mg of tissue.
KeywordsColumn liquid chromatography Fluorescence derivatization Intramolecular excimer Histamine in rat plasma and tissue 4-(1-Pyrene)butyric acid N-hydroxysuccinimide ester
This work was supported in part by a Grant-in-Aid for Scientific Research (C) (No. 14572043) from the Ministry of Education, Science, Sports and Culture of Japan, and Advanced Materials Institute, Fukuoka University.