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Chromatographia

, Volume 70, Issue 3–4, pp 575–580 | Cite as

Determination of Histamine in Rat Plasma and Tissue Extracts by Intramolecular Excimer-Forming Derivatization and LC with Fluorescence Detection

  • Fumio Ichinose
  • Takashi Yoshitake
  • Hideyuki Yoshida
  • Kenichiro Todoroki
  • Jan Kehr
  • Osamu Inoue
  • Hitoshi Nohta
  • Masatoshi YamaguchiEmail author
Full Short Communication

Abstract

A highly sensitive and selective method for determination of histamine in rat plasma and tissue extracts by liquid chromatography with fluorescence detection is described. The method is based on precolumn derivatization of amino groups of histamine with two molecules of 4-(1-pyrene)butyric acid N-hydroxysuccinimide ester that allows generation of intramolecular excimer fluorescence. The detection limit for histamine was 0.183 nM. This sensitivity allowed determination of histamine in 10 μL of rat plasma or in the extracts from less than 1 mg of tissue.

Keywords

Column liquid chromatography Fluorescence derivatization Intramolecular excimer Histamine in rat plasma and tissue 4-(1-Pyrene)butyric acid N-hydroxysuccinimide ester 

Notes

Acknowledgments

This work was supported in part by a Grant-in-Aid for Scientific Research (C) (No. 14572043) from the Ministry of Education, Science, Sports and Culture of Japan, and Advanced Materials Institute, Fukuoka University.

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Fumio Ichinose
    • 1
    • 2
  • Takashi Yoshitake
    • 3
    • 4
  • Hideyuki Yoshida
    • 1
  • Kenichiro Todoroki
    • 1
  • Jan Kehr
    • 3
  • Osamu Inoue
    • 5
  • Hitoshi Nohta
    • 1
  • Masatoshi Yamaguchi
    • 1
    Email author
  1. 1.Faculty of Pharmaceutical SciencesFukuoka UniversityFukuokaJapan
  2. 2.Chemicals Evaluation and Research InstituteHitaJapan
  3. 3.Department of Physiology and PharmacologyKarolinska InstitutetStockholmSweden
  4. 4.School of Pharmaceutical SciencesInternational University of Health and WelfareOhtawaraJapan
  5. 5.Department of Medical Physics, School of Allied Health Sciences, Faculty of MedicineOsaka UniversitySuitaJapan

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