Chromatographia

, Volume 69, Issue 9–10, pp 1129–1131 | Cite as

A Validated RP-LC Method for the Determination of Isosorbide 5-Mononitrate in a Leishmanicidal Ointment

  • Philippe Dallet
  • Tina Kauss
  • Marie-Hélène Langlois
  • Fawaz Fawaz
  • Djavad M. Mossalayi
  • Jean-Pierre Dubost
Limited Short Communication
First Online:
Received:
Revised:
Accepted:
  • 54 Downloads

Abstract

An RP-LC method using a YMC-Pack ODS-AQ column and UV detection (220 nm) was validated for the determination of isosorbide 5-mononitrate selected as an exogenous NO source in a leishmanicidal ointment. After extraction with hot water, the extracts were analysed at 35 °C using isocratic conditions (water-acetonitrile, 4:1 v/v; 0.9 mL min−1). The method was specific, precise, accurate and linear.

Keywords

Column liquid chromatography Validation Leishmaniasis Isosorbide 5-mononitrate 
This is a preview of subscription content, log in to check access.

References

  1. 1.
    Markle WH, Makhoul K (2000) Am Fam Phys 69:1455–1460Google Scholar
  2. 2.
    Mossalayi MD, Arock M, Mazier D, Vincendeau P, Vouldoukis I (1999) Parasitol Today 15:342–345. doi: 10.1016/S0169-4758(99)01477-5 CrossRefGoogle Scholar
  3. 3.
    Thatcher GRJ, Nicolescu AC, Bennett BM, Toader V (2004) Free Radic Biol Med 37:1122–1143. doi: 10.1016/j.freeradbiomed.2004.06.013 CrossRefGoogle Scholar
  4. 4.
    Buffet PA, Morizot G (2003) Bull Soc Pathol Exot 96:383–388Google Scholar
  5. 5.
    López-Jaramillo P, Ruano C, Rivera J, Terán E, Salazar-Irigoyen R, Esplugues JV, Moncada S (1998) Lancet 351:1176–1177. doi: 10.1016/S0140-6736(05)79119-4 CrossRefGoogle Scholar
  6. 6.
    Wang PG, Xian M, Tang X, Wu X, Wen Z, Janczuk AJ (2002) Chem Rev 102:1091–1134. doi: 10.1021/cr000040l CrossRefGoogle Scholar
  7. 7.
    Parker JD, Parker J (1998) N Engl J Med 338:520–531. doi: 10.1056/NEJM199802193380807 CrossRefGoogle Scholar
  8. 8.
    Verma RK, Garg S (2002) J Pharm Biomed Anal 30:583–591. doi: 10.1016/S0731-7085(02)00317-5 CrossRefGoogle Scholar
  9. 9.
    Gandhimathi M, Ravi TK, Abraham A, Thomas R (2003) J Pharm Biomed Anal 32:1145–1148. doi: 10.1016/S0731-7085(03)00248-6 CrossRefGoogle Scholar
  10. 10.
  11. 11.
    European Pharmacopoeia 6th edn (2008) European Council, Strasbourg, France, pp 2354–2356Google Scholar
  12. 12.

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Philippe Dallet
    • 1
  • Tina Kauss
    • 1
  • Marie-Hélène Langlois
    • 1
  • Fawaz Fawaz
    • 2
  • Djavad M. Mossalayi
    • 3
  • Jean-Pierre Dubost
    • 1
  1. 1.Laboratoire de Chimie AnalytiqueUniversité Victor Segalen Bordeaux 2Bordeaux cedexFrance
  2. 2.Laboratoire de Pharmacie GaléniqueUniversité Victor Segalen Bordeaux 2Bordeaux cedexFrance
  3. 3.Laboratoire d’Immunologie et de ParasitologieUniversité Victor Segalen Bordeaux 2Bordeaux cedexFrance

Personalised recommendations