Advertisement

Chromatographia

, Volume 69, Issue 7–8, pp 761–764 | Cite as

Confirmation and Determination of Sugars in Soft Drink Products by IEC with ESI-MS

  • Zuliang Chen
  • Xiaoyin Jin
  • Qinqping Wang
  • Yuman Lin
  • Li Gan
Full Short Communication

Abstract

In this paper, ion-exclusion chromatography with electrospray ionization mass spectrometry was investigated as an alternative method to confirm and determine sugar (glucose, fructose and sucrose) using volatile acidic eluent. Under the positive or negative ionization mode, the ESI-MS provided reasonable signals from positive ion mode as [M+H]+ and [M+NH4]+, or from negative ion mode as [M−H] and [M+Cl–H]. However, higher mass spectral intensity for ions was obtained under the positive mode. The separation of target sugars was achieved within 8 min using an eluent containing 0.1% (v/v) acetic acid. Linear plots of peak area versus concentration were obtained over the range 20–5,000 μg L−1. The detection limits of these compounds ranged from 10 to 15 μg L−1. The proposed method was used for the confirmation and quantification of sugars in soft drinks.

Keywords

Ion-exclusion chromatography Electrospray ionization mass spectrometry Soft drinks Sugars 

References

  1. 1.
    Adams MA, Chen ZL, Landman P, Comer TD (2005) Anal Biochem 264:77–84Google Scholar
  2. 2.
    Jensen MB, Johnson DC (1997) Anal Chem 69:1776–1781. doi: 10.1021/ac960828x CrossRefGoogle Scholar
  3. 3.
    Lee YC (1996) J Chromatogr A 720:137–149. doi: 10.1016/0021-9673(95)00222-7 CrossRefGoogle Scholar
  4. 4.
    Rocklin RD, Clarke AP, Weitzhandler M (1998) Anal Chem 70:1496–1501. doi: 10.1021/ac970906w CrossRefGoogle Scholar
  5. 5.
    Cheng J, Jandik P, Avdalovic N (2003) Anal Chem 75:572–579. doi: 10.1021/ac0204754 CrossRefGoogle Scholar
  6. 6.
    Clarke AP, Jandik J, Rocklin RD, Liu Y, Avdalovic N (1999) Anal Chem 71:2774–2781. doi: 10.1021/ac9811524 CrossRefGoogle Scholar
  7. 7.
    Yu H, Ding Y, Mou S, Jandik P, Cheng J (2002) J Chromatogr A 966:89–97. doi: 10.1016/S0021-9673(02)00739-2 CrossRefGoogle Scholar
  8. 8.
    Cai Y, Liua J, Shi Y, Liang L, Mou S (2005) J Chromatogr A 1085:98–103. doi: 10.1016/j.chroma.2004.11.100 CrossRefGoogle Scholar
  9. 9.
    Fischer K (2002) Anal Chim Acta 465:157–173. doi: 10.1016/S0003-2670(02)00204-0 CrossRefGoogle Scholar
  10. 10.
    Chen ZL, Adams MA (1999) Anal Chim Acta 386:249–256. doi: 10.1016/S0003-2670(99)00009-4 CrossRefGoogle Scholar
  11. 11.
    Chen ZL, Glod BK, Adams MA (1998) J Chromatogr A 818:61–68. doi: 10.1016/S0021-9673(98)00494-4 CrossRefGoogle Scholar
  12. 12.
    Chen ZL, Adams MA (1998) J Liq Chromatogr Relat Technol 21:24352–24445Google Scholar
  13. 13.
    Buttler T, Gordon L, Marko-Varga G (1993) Anal Chim Acta 279:27–37. doi: 10.1016/0003-2670(93)85063-P CrossRefGoogle Scholar
  14. 14.
    Chen ZL, Hibbert DB (1997) J Chromatogr A 766:27–33. doi: 10.1016/S0021-9673(96)00971-5 CrossRefGoogle Scholar
  15. 15.
    Rosen AL, Hieftje GM (2004) Spectrochim Acta, B At Spectrosc 59:135–146. doi: 10.1016/j.sab.2003.09.004 CrossRefGoogle Scholar
  16. 16.
    Reemtsma T (2003) J Chromatogr A 1000:477–501. doi: 10.1016/S0021-9673(03)00507-7 CrossRefGoogle Scholar
  17. 17.
    Bruggink C, Maurer R, Herrmann H, Cavalli S, Hoefler F (2005) J Chromatogr A 1085:104–109. doi: 10.1016/j.chroma.2005.03.108 CrossRefGoogle Scholar
  18. 18.
    Guignard G, Jouve ML, Bog’eat-Triboulot B, Dreyer E, Hausman JF, Hoffmann L (2005) J Chromatogr A 1085:137–142. doi: 10.1016/j.chroma.2005.05.068 CrossRefGoogle Scholar
  19. 19.
    Cheng CY, Tsai HR, Chang KC (2006) J Chromatogr A 1119:188–196. doi: 10.1016/j.chroma.2005.11.115 CrossRefGoogle Scholar
  20. 20.
    Wan ECH, Yu JZ (2006) J Chromatogr A 1107:175–181. doi: 10.1016/j.chroma.2005.12.062 CrossRefGoogle Scholar
  21. 21.
    Gamoh K, Saitoh H, Wada H (2003) Rapid Commun Mass Spectrom 17:685–689. doi: 10.1002/rcm.971 CrossRefGoogle Scholar
  22. 22.
    Tanaka K, Fritz JS (1986) J Chromatogr A 361:151–159. doi: 10.1016/S0021-9673(01)86902-8 CrossRefGoogle Scholar
  23. 23.
    Tanaka K, Ohta K, Fritz JS, Lee YS, Shim SB (1995) J Chromatogr A 706:385–393. doi: 10.1016/0021-9673(95)00041-K CrossRefGoogle Scholar
  24. 24.
    Glod BK, Baumann M (2003) J Sep Sci 26:1547–1553. doi: 10.1002/jssc.200301559 CrossRefGoogle Scholar
  25. 25.
    DIONEX, “Determination of sugar alcohols in confections and fruit juices by high-performance anion-exchange chromatography with pulsed amperometric detection” Application note 87, http://www.dionex.com

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Zuliang Chen
    • 1
  • Xiaoyin Jin
    • 1
  • Qinqping Wang
    • 1
  • Yuman Lin
    • 1
  • Li Gan
    • 1
  1. 1.School of Chemistry and Materials ScienceFujian Normal UniversityFuzhouChina

Personalised recommendations