Optimization of the LC Separation of 15 Phenolic Compounds with Plackett–Burman Designs
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In this study Plackett–Burman designs have been used to find the optimum conditions for separation of 15 phenolic compounds by LC. The responses used were the minimum resolution between two peaks and the analysis time. The adopted factors and experimental field were organic modifier (MeOH or ACN), percentage of acetic acid in the aqueous phase, flow rate, gradient with ten steps of percentage increase of the organic component of the mobile phase, and ten factors representing the duration of the steps. Use of Plackett–Burman designs achieved separation of the phenolic compounds within 45 min.
KeywordsColumn liquid chromatography Olive oil Polyphenols Plackett–Burman designs Experimental designs Screening
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