Chromatographia

, Volume 68, Issue 11–12, pp 1033–1038 | Cite as

Simultaneous Determination of Twelve Saponins in Radix et Rhizoma Notoginseng by Rapid Resolution LC-ESI-TOF-MS

  • Hai-Ling Liu
  • Li Xia
  • Jun Cao
  • Ping Li
  • Lian-Wen Qi
Full Short Communication
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Abstract

Rapid resolution liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry was developed for simultaneous determination of twelve saponins in Radix et Rhizoma Notoginseng (RRN) with macranthoidine A as internal standard. All calibration curves showed good linear regression (r 2 > 0.9935) within test range. The limits of detection of the twelve compounds were in the range of 1.53–5.08 ng mL−1. This method was successfully applied to analyze the saponins in fifteen samples of RRN.

Keywords

Column liquid chromatography Electrospray ionization time-of-flight mass spectrometry Saponins Radix et Rhizoma Notoginseng Panax notoginseng 
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Notes

Acknowledgments

This research was financially supported by the key program (No. 30530870) of the National Science Foundation of China.

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Hai-Ling Liu
    • 1
  • Li Xia
    • 1
  • Jun Cao
    • 1
  • Ping Li
    • 1
  • Lian-Wen Qi
    • 1
  1. 1.Key Laboratory of Modern Chinese MedicinesChina Pharmaceutical University, Ministry of EducationNanjingChina

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