Advertisement

Chromatographia

, 68:721 | Cite as

Simultaneous Determination of Atenolol and Chlorthalidone by LC–MS–MS in Human Plasma

  • Arshad Khuroo
  • Sanjeev Mishra
  • Onkar SinghEmail author
  • Saurabh Saxena
  • Tausif Monif
Original

Abstract

A simple, sensitive, selective and rapid liquid chromatography–tandem mass spectrometry method was developed and validated for the simultaneous separation and quantitation of atenolol and chlorthalidone in human plasma using metoprolol and hydrochlorothiazide as internal standard. Following solid phase extraction, the analytes were separated by an isocratic mobile phase on a reversed-phase C18 column and analyzed by MS in the multiple reaction-monitoring mode (atenolol in positive and chlorthalidone in the negative ion mode). The limit of quantitation for this method was 10 and 15 ng mL−1 and the linear dynamic range was generally 10–2,050 ng mL−1 and 15–3,035 ng mL−1 for atenolol and chlorthalidone, respectively.

Keywords

Column liquid chromatography–mass spectrometry Atenolol and chlorthalidone in human plasma 

Notes

Acknowledgments

The authors wish to acknowledge the support and facilities received from Ranbaxy Research Laboratories, Gurgaon, India.

References

  1. 1.
    Klag MJ, Whelton PK, Randall BL, Neaton JD, Brancati FL, Ford CE et al (1996) N Engl J Med 334:13–18. doi: 10.1056/NEJM199601043340103 CrossRefGoogle Scholar
  2. 2.
    Coresh J, Wei GL, McQuillan G, Brancati FL, Levey AS, Jones C et al (2001) Arch Intern Med 161(9):1207–1216. doi: 10.1001/archinte.161.9.1207 CrossRefGoogle Scholar
  3. 3.
    Kearney PM, Whelton M, Reynolds K, Muntner P, Whelton PK, He J (2005) Lancet 365(9455):217–223Google Scholar
  4. 4.
    Lewington S, Clarke R, Qizilbash N, Peto R, Collins R (2002) Lancet 360(9349):1903–1913. doi: 10.1016/S0140-6736(02)11911-8 CrossRefGoogle Scholar
  5. 5.
    Johnson RD, Lewis RJ (2006) Forensic Sci Int 156(2–3):106–117. doi: 10.1016/j.forsciint.2005.01.001 CrossRefGoogle Scholar
  6. 6.
    Medical Research Council Working Party on Mild to Moderate Hypertension (1992) Medical research trial on treatment of hypertension in older adults: principal results. BMJ 304:405–412CrossRefGoogle Scholar
  7. 7.
    Siscovick DS, Raghunathan TE, Psaty BM, Koepsell TD, Wicklund KG, Lin X et al (1994) N Engl J Med 330:1852–1857. doi: 10.1056/NEJM199406303302603 CrossRefGoogle Scholar
  8. 8.
    Collins R, Peto R, MacMahon S, Hebert P, Fiebach NH, Eberlein KA et al (1990) Lancet 335:827–838. doi: 10.1016/0140-6736(90)90944-Z CrossRefGoogle Scholar
  9. 9.
    Sica DA (1994) Drugs 48:16–24. doi: 10.2165/00003495-199448010-00003 CrossRefGoogle Scholar
  10. 10.
    Neutel JM, Black HR, Weber MA (1996) Am J Med 101:61–70. doi: 10.1016/S0002-9343(96)00269-0 CrossRefGoogle Scholar
  11. 11.
    Kataoka H, Lord HL, Pawliszyn J (1999) Anal Chem 71:4237–4244. doi: 10.1021/ac990356x CrossRefGoogle Scholar
  12. 12.
    Abdel-Hamid ME (2000) Farmaco 55(2):136–145. doi: 10.1016/S0014-827X(00)00006-9 CrossRefGoogle Scholar
  13. 13.
    Johnson R, Lewis R (2006) Forensic Sci Int 156(2):106–117. doi: 10.1016/j.forsciint.2005.01.001 CrossRefGoogle Scholar
  14. 14.
    Ervik M, Gustavii K (1974) Anal Chem 46:39–42. doi: 10.1021/ac60337a046 CrossRefGoogle Scholar
  15. 15.
    Degen PH, Schweizer A (1977) J Chromatogr A 142:549–557. doi: 10.1016/S0021-9673(01)92067-9 CrossRefGoogle Scholar
  16. 16.
    Rosenberg MJ, Lam KK, Dorsey TE (1986) J Chromatogr A 375:438Google Scholar
  17. 17.
    MacGregor R, Farina PR, Hagopian M, Hay N, Esber HJ, Jkeirns J (1984) Ther Drug Monit 6:83. doi: 10.1097/00007691-198403000-00014 CrossRefGoogle Scholar
  18. 18.
    Dadgar D, Kelly MT (1988) Analyst Lond 113:113. doi: 10.1039/an9881300113 CrossRefGoogle Scholar
  19. 19.
    Muirhead DC, Christie RB (1987) J Chromatgr 416:420. doi: 10.1016/0378-4347(87)80530-3 CrossRefGoogle Scholar
  20. 20.
    Guidance for industry: bioanalytical method validation, US department of health and human services, food and drug administration centre for drug evaluation and research (CDER), centre for veterinary medicine (CBM), May 2001Google Scholar
  21. 21.
    FDA guidance for industry: bioavailability studies for orally administered drug-products- general considerations, US department of health and human services, food and drug administration centre for drug evaluation and research (CDER) 2000Google Scholar

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Arshad Khuroo
    • 1
  • Sanjeev Mishra
    • 1
  • Onkar Singh
    • 1
    Email author
  • Saurabh Saxena
    • 1
  • Tausif Monif
    • 1
  1. 1.Clinical Pharmacology and PharmacokineticsGurgaonIndia

Personalised recommendations