Chromatographia

, 68:179

Chromatographic Framework to Determine the Memantine Binding Mechanism on Human Serum Albumin Surface

  • Firas Ibrahim
  • Yves-Claude Guillaume
  • Claire André
Original

DOI: 10.1365/s10337-008-0675-6

Cite this article as:
Ibrahim, F., Guillaume, YC. & André, C. Chroma (2008) 68: 179. doi:10.1365/s10337-008-0675-6

Abstract

In this work, the interaction of memantine with human serum albumin (HSA) immobilized on porous silica particles was studied using a biochromatographic approach. The determination of the enthalpy change at different pH values suggested that the protonated group in the memantine–HSA complex exhibits a heat protonation with a magnitude around 65 kJ mol−1. This value agrees with the protonation of a guanidinium group, and confirmed that an arginine group may become protonated in the memantine–HSA complex formation. The thermodynamic data showed that memantine–HSA binding, for low temperature (<293 K), is dominated by a positive entropy change. This result suggests that dehydration at the binding interface and charge–charge interactions contribute to the memantine–HSA complex formation. Above 293 K, the thermodynamic data ΔH and ΔS became negative due to van der Waals interactions and hydrogen bonding which are engaged at the complex interface. The temperature dependence of the free energy of binding is weak because of the enthalpy–entropy compensation caused by a large heat capacity change, ΔCp = − 3.79 kJ mol−1 K−1 at pH = 7. These results were used to determine the potential binding site of this drug on HSA.

Keywords

Column liquid chromatography Human serum albumin Memantine 

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Firas Ibrahim
    • 1
  • Yves-Claude Guillaume
    • 1
  • Claire André
    • 1
  1. 1.Equipe des Sciences Séparatives et Biopharmaceutiques (2SB/EA-3924), Laboratoire de Chimie Analytique, Faculté de Médecine PharmacieUniversité de Franche-ComtéBesançon CedexFrance

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