Chromatographia

, Volume 68, Issue 5–6, pp 431–435 | Cite as

Quantitative Analysis of Genistein in Human Plasma by Online Concentration CE with UV Detection

  • Hai-Yan Feng
  • Shu-Rong Hou
  • Na Zheng
  • Xiang-Jun Li
  • Zhong-Bo Hu
  • Zhuo-Bin Yuan
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Abstract

A rapid capillary electrophoresis method based on online acid barrage stacking has been successfully established for analysis of trace amounts of genistein in human plasma. Genistein was analyzed within 8 min, with 200 mmol L−1 citric acid (pH 1.7) as acid barrage, and injection times of 180 and 30 s for sample and acid, respectively. Good linearity was obtained in the range 0.05–5 mg L−1 and the limit of detection was 0.03 mg L−1. Compared with normal sample injection, this method resulted in more than a fiftyfold improvement in detection sensitivity. The technique has potential for use in studies of genistein metabolism and of exposure levels in humans.

Keywords

Capillary electrophoresis Online acid barrage stacking Genistein Plasma 
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Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 20235010, 20775088), the Foundation of State Key Laboratory of Electroanalytical Chemistry (No. 2006002), and the President Foundation of GUCAS.

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Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Hai-Yan Feng
    • 1
    • 2
  • Shu-Rong Hou
    • 1
  • Na Zheng
    • 1
  • Xiang-Jun Li
    • 1
  • Zhong-Bo Hu
    • 1
  • Zhuo-Bin Yuan
    • 1
  1. 1.College of Chemistry and Chemical EngineeringThe Graduate University of the Chinese Academy of SciencesBeijingChina
  2. 2.School of Chemical EngineeringShijiazhuang UniversityShijiazhuangChina

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