Chromatographia

, Volume 67, Issue 11–12, pp 923–927 | Cite as

New Mab CB.Hep-1 Purification Process Eliminates the Need for Pre-Chromatographic Purification. Stability Demonstrated Over 100 Purification Cycles

  • Rodolfo Valdés
  • Tatiana Álvarez
  • Andrés Tamayo
  • Eutimio G. Fernandez
  • José Montero
  • Déborah Geada
  • William Ferro
  • Yenisley Medina
  • Yarysel Guevara
  • Sigifredo Padilla
  • Dobián Cecilia
  • Lamay Dorta
  • Marcos González
  • Raudel Sosa
  • Tatiana González
Full Short Communication
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Abstract

This investigation sought to discover whether purification of monoclonal antibody CB.Hep-1 in ascitic fluid is possible by protein A–Sepharose affinity chromatography, for 100 runs, without pre-purification steps. Results showed that direct application of ascitic fluid to protein A–Sepharose increased monoclonal antibody recovery by 27% compared with the traditional process (control) after 100 runs. The purity of the monoclonal antibody was >95% and the cost of the purification was 15% less than that of the control process. In conclusion, monoclonal antibody CB.Hep-1 in ascitic fluid can be purified by chromatography on protein A–Sepharose, for 100 purification cycles, without the need for pre-purification steps.

Keywords

Affinity chromatography Monoclonal antibody Protein purification Protein A–Sepharose 
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Notes

Acknowledgments

The authors would like to thank to the Monoclonal Antibody Department of the Center for Genetic Engineering and Biotechnology for their contribution to this comparison study. The authors also gratefully acknowledge Mena Cayetana Ramos from ‘Salvador Allende’ hospital for her effort to make possible the publication of this manuscript.

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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Rodolfo Valdés
    • 1
  • Tatiana Álvarez
    • 1
  • Andrés Tamayo
    • 1
  • Eutimio G. Fernandez
    • 2
  • José Montero
    • 1
  • Déborah Geada
    • 3
  • William Ferro
    • 1
  • Yenisley Medina
    • 1
  • Yarysel Guevara
    • 1
  • Sigifredo Padilla
    • 1
  • Dobián Cecilia
    • 1
  • Lamay Dorta
    • 1
  • Marcos González
    • 1
  • Raudel Sosa
    • 4
  • Tatiana González
    • 4
  1. 1.Monoclonal Antibody Production DepartmentCenter for Genetic Engineering and BiotechnologyHavanaCuba
  2. 2.Inorganic Chemistry DepartmentCenter for Engineering and Chemical ResearchHavanaCuba
  3. 3.Tobacco Research InstituteHavanaCuba
  4. 4.Process Control DepartmentCenter for Genetic Engineering and BiotechnologyHavanaCuba

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