Efficient and Sensitive Method for Quantitative Determination and Validation of Umbelliferone, Carvone and Myristicin in Anethum graveolens and Carum carvi Seed
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An efficient HPTLC method for the analysis of umbelliferone, carvone and myristicin in Anethum graveolens and Carum carvi seed was developed. The method employed HPTLC plates precoated with silica gel 60 F254 as the stationary phase. Methanol extracts of seeds from three different sources were used. The calibration plot for umbelliferone, carvone and myristicin were linear with the correlation coefficient of 0.997 ± 0.016, 0.999 ± 0.009 and 0.999 ± 0.013, respectively, which were indicative of good linear dependence of peak area on concentration. The method permits reliable quantification of umbelliferone, carvone and myristicin and showed good resolution and separation. The method was validated as per ICH guidelines. To study the accuracy of the method, recovery studies were performed by the method of standard addition at three different levels and the average percentage recovery was found to be 99.05% for umbelliferone, 100.28% for carvone and 99.8% for myristicin. The proposed HPTLC method for quantitative monitoring of umbelliferone, carvone and myristicin in A. graveolens and C. carvi seed can be used for routine quality testing of these extracts.
KeywordsUmbelliferones carvone and myristicin Thin layer chromatography Anethum graveolens and Carum carvi Seed
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