Chromatographia

, Volume 66, Issue 7–8, pp 487–491 | Cite as

HPLC Determination of Lovastatin in Rat Tissue

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Abstract

A simple HPLC method has been developed and validated for the determination of lovastatin in rat tissues. Samples were prepared by a simple protein precipitation. Separation was carried out on a C18 column with a mobile phase of acetonitrile:0.05 M ammonium acetate, a flow rate of 1.0 mL min−1 and with detection at 238 nm. There was no interference from endogenous tissue compounds. The calibration curve was linear from 0.0175 to 7.0 μg mL−1 with a limit of detection of 0.006 μg mL−1. The method was used to measure the concentration of lovastatin in rat tissue after a single oral dose. The highest level was observed in the liver, then in kidney, heart and spleen; the lowest level was found in the brain. These results suggest that lovastatin distributes rapidly into all tissues and particularly the liver.

Keywords

Column liquid chromatography Rat tissues Lovastatin Drug distribution 
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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  1. 1.Department of PharmacyNorth China Coal Medical UniversityTangshanPeople’s Republic of China
  2. 2.Institute of Medicinal BiotechnologyPeking Union Medical College and Chinese Academy of Medical SciencesBeijingPeople’s Republic of China

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