, Volume 66, Issue 3–4, pp 185–190 | Cite as

Comparison of UPLC and HPLC for Determination of trans-10-Hydroxy-2-Decenoic Acid Content in Royal Jelly by Ultrasound-Assisted Extraction with Internal Standard

  • Jinhui Zhou
  • Jing Zhao
  • Hancheng Yuan
  • Ying Meng
  • Yi Li
  • Liming Wu
  • Xiaofeng Xue


The ultra performance liquid chromatography (UPLC) and high performance liquid chromatography (HPLC) method was developed and compared to detect the trans-10-hydroxy-2-decenoic acid (10-HDA) content in royal jelly cream and lyophilized powder. The sample was extracted using absolute ethanol based on the ultrasound-assisted extraction. Chromatographic separation of 10-HDA and methyl 4-hydroxybenzoate as internal standard was carried out on a Nova-pak® C18 column for HPLC and on Acquity BEH C18 column for UPLC. In the HPLC system, the average recoveries were 95.0–99.2% (= 5) with relative standard deviation (RSD) values of 1.1–2.1% for royal jelly cream and 98.0–100.0% (= 5) with RSD values of 1.6–3.0% for lyophilized powder, respectively. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.5 and 1.5 mg kg−1 for both royal jelly cream and lyophilized powder. In the UPLC system, the limit of detection (LOD) and limit of quantitation (LOQ) were 0.3 and 1.0 mg kg−1 for both royal jelly cream and lyophilized powder. The major advantages of UPLC, using 1.7 μm particles, over HPLC are the speed of analysis, the narrower peaks and the decrease of solvent consumption for the 10-HDA in the analyses of beeproducts. The two methods were validated for the determination of practical royal jelly products. The concentration of 10-HDA ranges from 1.26 to 2.25% for pure royal jelly cream samples and 3.01–6.26% for royal jelly lyophilized powder samples. For 30 royal jelly products, the 10-HDA content varied from no detectable to 1.005%.


Column liquid chromatography trans-10-Hydroxy-2-decenoic acid Ultrasound-assisted extraction Royal jelly cream Lyophilized powder 


  1. 1.
    Münstedt K, von Georgi R (2003) Am Bee J 43:647–650Google Scholar
  2. 2.
    Wang GY, Lin ZB (1996) Chin J Pharm Toxicol 1:53–55Google Scholar
  3. 3.
    Rembold H (1965) Vitam Horm 23:359–382CrossRefGoogle Scholar
  4. 4.
    Iannuzzi J (1990) Am Bee J 130:587–589Google Scholar
  5. 5.
    Inoue SI, Koya-Miyata S, Ushio S, Iwaki K, Ikeda M, Kurimoto M (2003) Exp Gerontol 38:965–969CrossRefGoogle Scholar
  6. 6.
    Dixit PK, Patel NG (1964) Nature 202:189–190CrossRefGoogle Scholar
  7. 7.
    Kramer KJ, Tager HS, Childs CN, Speirs RD (1977) J Insect Physiol 23:293–295CrossRefGoogle Scholar
  8. 8.
    Fujii A, Kobayashi S, Kuboyama N, Furukawa Y, Kaneko Y, Ishihama S, Yamamoto H, Tamura T (1990) Jpn J Pharmacol 53:331–337Google Scholar
  9. 9.
    Townsend GF, Morgan JF, Hazlett B (1959) Nature 183:1270–1271CrossRefGoogle Scholar
  10. 10.
    Townsend GF, Morgan JF, Tolnai S, Hazlett B, Morton HJ, Shuel RW (1960) Cancer Res 20:503–510Google Scholar
  11. 11.
    Barker SA, Foster AB, Lamb DC, Hodgson N (1959) Nature 183:996–997CrossRefGoogle Scholar
  12. 12.
    The Ministry of Agriculture of P.R.C. (2002) Nuisanceless food—the quality standard of royal jelly and lyophilized powderGoogle Scholar
  13. 13.
    Bloodworth BC, Harn CS, Hock CT (1995) J AOAC Int 78:1019–1023Google Scholar
  14. 14.
    Dai F, Li L (1990) Chin J Pharm Anal 10:108–109Google Scholar
  15. 15.
    Zhang X, Fu CY, Yang RF (1989) Chem J Chin Univ 10:609–613Google Scholar
  16. 16.
    Genc. M, Aslan A (1999) J Chromatogr A 839:265–268CrossRefGoogle Scholar
  17. 17.
    Long ZX, Wan CH, Wang K, Liu BP, Hu HS (2005) Chin Food Sci 26:170–172Google Scholar
  18. 18.
    Chen XP, Ni L, Zhao DH (2005) Pract Prev Med 12:172–172Google Scholar
  19. 19.
    Swartz ME (2005) LC-GC North Am 23:8–14Google Scholar
  20. 20.
    Ultra Performance LCTM by design, Waters Corporation, USA, 720000880EN LL&LW-UL (2004)Google Scholar
  21. 21.
    Luque de Castro MD, Da Silva MP (1997) Trends Analyt Chem 16:16–24CrossRefGoogle Scholar
  22. 22.
    Pacakova V, Štulik K, Jiskra J (1996) J Chromatogr A 754:17–31CrossRefGoogle Scholar
  23. 23.
    Hercegova A, Domotorova M, Matisova E, Kirchner M, Otrekal R, Stefuca V (2005) J Chromatogr A 1084:46–53CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  • Jinhui Zhou
    • 1
    • 2
  • Jing Zhao
    • 1
    • 2
  • Hancheng Yuan
    • 3
  • Ying Meng
    • 3
  • Yi Li
    • 1
    • 2
  • Liming Wu
    • 1
    • 2
  • Xiaofeng Xue
    • 1
    • 2
  1. 1.Bee Research InstituteChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  2. 2.Beeproduct Quality Supervision and Testing CenterMinistry of AgricultureBeijingPeople’s Republic of China
  3. 3.Waters Technologies Limited CorporationBeijingPeople’s Republic of China

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