, Volume 66, Supplement 1, pp 129–133 | Cite as

Simultaneous LC–MS–MS Determination of Zolmitriptan and Its Active Metabolite N-Desmethylzolmitriptan in Human Plasma

  • B. KılıçEmail author
  • T. Özden
  • S. Toptan
  • S. Özilhan
Full Short Communication


A specific and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry method was developed for the determination of zolmitriptan and N-desmethylzolmitriptan in human plasma. The analytes and the internal standard (IS) paroxetine were extracted by liquid–liquid extraction with a mixture of saturated ethyl acetate:dichloromethane (4:1) and were separated using an isocratic mobile phase on a XTerra RP18 column. The mobile phase used was acetonitrile: 5 mM ammonium acetate: formic acid (50:50:0.053, v/v/v). Zolmitriptan and N-desmethylzolmitriptan in a range of 0.25–20 ng mL−1 were easily quantified. The validated method can be applied to pharmacokinetic and bioequivalence studies.


Column liquid chromatography–mass spectrometry LC–MS–MS Pharmacokinetics Human plasma Zolmitriptan N-Desmethylzolmitriptan 


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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  • B. Kılıç
    • 1
    Email author
  • T. Özden
    • 2
  • S. Toptan
    • 1
  • S. Özilhan
    • 1
  1. 1.Novagenix Bioanalytical R&D CentreAnkaraTurkey
  2. 2.Faculty of PharmacyGazi UniversityAnkaraTurkey

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