Advertisement

Chromatographia

, Volume 66, Supplement 1, pp 129–133 | Cite as

Simultaneous LC–MS–MS Determination of Zolmitriptan and Its Active Metabolite N-Desmethylzolmitriptan in Human Plasma

  • B. KılıçEmail author
  • T. Özden
  • S. Toptan
  • S. Özilhan
Full Short Communication

Abstract

A specific and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry method was developed for the determination of zolmitriptan and N-desmethylzolmitriptan in human plasma. The analytes and the internal standard (IS) paroxetine were extracted by liquid–liquid extraction with a mixture of saturated ethyl acetate:dichloromethane (4:1) and were separated using an isocratic mobile phase on a XTerra RP18 column. The mobile phase used was acetonitrile: 5 mM ammonium acetate: formic acid (50:50:0.053, v/v/v). Zolmitriptan and N-desmethylzolmitriptan in a range of 0.25–20 ng mL−1 were easily quantified. The validated method can be applied to pharmacokinetic and bioequivalence studies.

Keywords

Column liquid chromatography–mass spectrometry LC–MS–MS Pharmacokinetics Human plasma Zolmitriptan N-Desmethylzolmitriptan 

References

  1. 1.
    Rapoport AM, Cady RK, Mathew NT (1995) Cephalalgia 15:221–226Google Scholar
  2. 2.
    Seaber E, On N, Dixon RM (1997) Br J Clin Pharmacol 43:579–587CrossRefGoogle Scholar
  3. 3.
    Chen J, Jiang XG, Jiang WM, Mei N, Gao XL, Zhang QZ (2004) J Pharm Biomed Anal 35:639–645CrossRefGoogle Scholar
  4. 4.
    Clement EM, Franklin M (2002) J Chromatogr B 766:339–343CrossRefGoogle Scholar
  5. 5.
    Vishwanathan K, Barlett MG, Stewart JT (2000) Rapid Commun Mass Spectrom 14:168–172CrossRefGoogle Scholar
  6. 6.
    Zhang Z, Xu F, Tian Y, Li W, Mao G (2004) J Chromatogr B 813:227–233CrossRefGoogle Scholar
  7. 7.
    Chen X, Liu D, Luan Y, Jin F, Zhong D (2006) J Chromatogr B 832:30–35CrossRefGoogle Scholar
  8. 8.
    Karnes HT, Shiu G, Shah VP (1991) Pharm Res 8:421–426CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  • B. Kılıç
    • 1
    Email author
  • T. Özden
    • 2
  • S. Toptan
    • 1
  • S. Özilhan
    • 1
  1. 1.Novagenix Bioanalytical R&D CentreAnkaraTurkey
  2. 2.Faculty of PharmacyGazi UniversityAnkaraTurkey

Personalised recommendations