Simultaneous LC–MS–MS Determination of Zolmitriptan and Its Active Metabolite N-Desmethylzolmitriptan in Human Plasma
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A specific and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry method was developed for the determination of zolmitriptan and N-desmethylzolmitriptan in human plasma. The analytes and the internal standard (IS) paroxetine were extracted by liquid–liquid extraction with a mixture of saturated ethyl acetate:dichloromethane (4:1) and were separated using an isocratic mobile phase on a XTerra RP18 column. The mobile phase used was acetonitrile: 5 mM ammonium acetate: formic acid (50:50:0.053, v/v/v). Zolmitriptan and N-desmethylzolmitriptan in a range of 0.25–20 ng mL−1 were easily quantified. The validated method can be applied to pharmacokinetic and bioequivalence studies.
KeywordsColumn liquid chromatography–mass spectrometry LC–MS–MS Pharmacokinetics Human plasma Zolmitriptan N-Desmethylzolmitriptan
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