Chromatographia

, Volume 65, Issue 5–6, pp 305–311 | Cite as

Fast CE Determination of d-Amphetamine and Diphenhydramine in Quick-Acting Anti-Motion Capsules

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Abstract

The quick separation and simultaneous determination of d-amphetamine and diphenhydramine in the quick-acting anti-motion capsules was investigated by capillary zone electrophoresis. The influence of different parameters (internal standard, injection modes, pH, concentration of the running buffer and applied voltage) was systematically studied. The two compounds could be well separated within 2.0 min in a 40.2 cm fused-silica capillary at a separation voltage of 20 kV in a 50 mM phosphate–12.5 mM borate buffer adjusted to pH 5.5. Correlation coefficients for calibration curves in the range 0.50–1.50 μg mL−1 for d-amphetamine and 2.75–8.25 μg mL−1 for diphenhydramine were higher than 0.999. The limits of detection of d-amphetamine and diphenhydramine were 10.0 and 5.5 ng mL−1 and the recoveries of the compounds in the QAAMC were 99.80 and 99.85%, respectively.

Keywords

Capillary zone electrophoresis Quick-action anti-motion capsules Motion sickness prevention d-Amphetamine and diphenhydramine 
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Notes

Acknowledgements

This work was supported by Fundamental Research Key Project founded by Science & Technology department of Shanghai, China, Grant NO. 03JC14005.

References

  1. 1.
    Gahlinger PM (1999) Postgrad Med 106:177–184CrossRefGoogle Scholar
  2. 2.
    Benson AJ (1998) In: Stellman JM (ed) Encyclopaedia of occupational health and safety, 4th edn. International Labour Office, Geneva 50 pp 12–4Google Scholar
  3. 3.
    Yin L, Li M, Wang HM (2004) Zhong Hua Hang Hai Yi Xue Yu Gao Qi Ya Yi Xue Za Zhi 11:122–123Google Scholar
  4. 4.
    Stadelmann WK, Reintgen DS (1998) Hematol Oncol Clin North Am 12:767–796CrossRefGoogle Scholar
  5. 5.
    Wang HM, Cao ZH, Zhang L, Xiao JP, Bao DH, Zheng LY, Ji HG, Pan XF (2000) Di Er Jun Yi Da Xue Xue Bao 21:745–748Google Scholar
  6. 6.
    Philipova D, Tzenova B, Iwanowitsch A, Bognar-Steinberg I (2004) Int J Clin Pharmacol Ther 42:218–231Google Scholar
  7. 7.
    Fan ZP, Su FQ, Zeng KR (2003) Hai Jun Yi Xue Za Zhi 23:291–293Google Scholar
  8. 8.
    Takeda N, Morita M, Horii A, Nishiike S, Kitanara T, Uno A (2001) J Med Invest 48:44–59Google Scholar
  9. 9.
    Wood CD, Graybiel A (1968) Aerosp Med 39:1341–1344Google Scholar
  10. 10.
    Asghar SJ, Baker GB, Rauw GA, Silverstone PH (2002) J Pharmacol Toxicol Meth 46:111–115CrossRefGoogle Scholar
  11. 11.
    Yoo SD, Axelson JE, Rurak DW (1986) J Chromatogr 378:385–393Google Scholar
  12. 12.
    Marquet P, Lacassie E, Battu C, Faubert H (1997) J Chromatogr B 700:77–82Google Scholar
  13. 13.
    March C, Karnes HT, Mclean A, Mukherjee PS (2001) Biomed Chromatogr 15:100–107CrossRefGoogle Scholar
  14. 14.
    AlDirbashi OY, Ikeda K, Takahashi M, Kuroda N, Ikeda S, Nakashima K (2001) Biomed Chromatogr 15:457–463CrossRefGoogle Scholar
  15. 15.
    Selinger K, Prevost J, Hill HM (1990) J Chromatogr 526:597–602Google Scholar
  16. 16.
    Skofitsch G, Lembeck F (1983) Arzneim Forsch 33:1674–1676Google Scholar
  17. 17.
    Han Y, Chen XY, Xie ZY, Zhong DF (2003) Yao Xue Xue Bao 38:67–70Google Scholar
  18. 18.
    Kumar S, Rurak DW, Riggs KW (1998) J Mass Spectrom 33:1171–1181CrossRefGoogle Scholar
  19. 19.
    Fuh MR, Haung CH, Wu TY, Lin SL, Pan WH (2004) Rapid Commun Mass Spectrom 15:1711–1714CrossRefGoogle Scholar
  20. 20.
    Hendrickson HP, Milesi-Halle A, Laurenzana EM, Owens SM (2004) J Chromatogr B 806:81–87CrossRefGoogle Scholar
  21. 21.
    Nieddu M, Boatto G, Carta A, Sanna A, Pisano M (2005) Biomed Chromatogr 19:737–742CrossRefGoogle Scholar
  22. 22.
    Boatto G, Faedda MV, Pau A, Asproni B, Menconi S, Cerri R (2002) J Pharm Biomed Anal 29:1073–1080CrossRefGoogle Scholar
  23. 23.
    Gomez MR, Sombra L, Olsina RA, Martinez LD, Silva MF, (2005) Farmaco 60:85–90CrossRefGoogle Scholar
  24. 24.
    Validation of analytical procedures: test and methodology, ICH Harmonised Tripartite Guideline, IFPMA Ed., (Geneva), 1994Google Scholar
  25. 25.
    Lee TT, Yeung ES (1992) Anal Chem 64:1226–1231CrossRefGoogle Scholar
  26. 26.
    Quirino JP, Terabe S (2000) Electrophoresis 21:355–359CrossRefGoogle Scholar
  27. 27.
    Galli V, Barbas C (2003) Anal Chim Acta 482:37–45CrossRefGoogle Scholar
  28. 28.
    Zhang LY, Sun MX (2004) J Chromatogr A 1040:133–140CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2006

Authors and Affiliations

  1. 1.Department of Pharmaceutical Analysis, School of PharmacySecond Military Medical UniversityShanghaiPeople’s Republic of China
  2. 2.Shanghai Key Laboratory for Pharmaceutical Metabolite ResearchShanghaiPeople’s Republic of China
  3. 3.Shanghai Institute for Drug ControlShanghaiPeople’s Republic of China

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